H2O2 oxidative preparation, characterization and antiradical activity of a novel oligosaccharide derived from flaxseed gum

Abstract

n this present study, a novel flaxseed gum oligosaccharide (FGOS) was prepared by H2O2 oxidative degradation method followed by physicochemical properties characterization and antiradical activity evaluation. Results indicated that the degradation rate of flaxseed gum is 37.81% under the optimum conditions (i.e., reaction temperature of 120 °C, H2O2 concentration of 0.2 M and reaction time of 2.0 h) and FGOS as a reddish brown semisolid was obtained. Physicochemical properties identification showed that FGOS has a molecular weight of 1047 Da and is a typical oligosaccharide which contains uronic acid. Characterizations showed FGOS is acid glycopyranose that consists of rhamnose, fucose, xylose, mannose, arabinose, glucose and galactose with mole percentages of 8.26%, 7.54%, 12.85%, 7.93%, 29.31%, 14.28% and 19.82% respectively. FGOS exhibited good free radical scavenging ability (OH 82.58%, DPPH 52.74% and ABTS 91.29% at most, respectively), suggesting its potent antiradical activity.