670nm light treatment following retinal injury modulates Muller cell gliosis: Evidence from in vivo and in vitro stress models
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Lu, Yen-Zhen (Angel); Fernando, Nilisha; Natoli, Riccardo; Madigan, Michele; Valter, Krisztina
Description
Photobiomodulation (PBM) with 670 nm light has been shown to accelerate wound healing in soft tissue injuries, and also to protect neuronal tissues. However, little data exist on its effects on the non-neuronal components of the retina, such as Miller cells (MCs), which are the principal macroglia of the retina that play a role in maintaining retinal homeostasis. The aim of this study was to explore the effects of 670 nm light on activated MCs using in vivo and in 141 TO stress models. Adult...[Show more]
dc.contributor.author | Lu, Yen-Zhen (Angel) | |
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dc.contributor.author | Fernando, Nilisha | |
dc.contributor.author | Natoli, Riccardo | |
dc.contributor.author | Madigan, Michele | |
dc.contributor.author | Valter, Krisztina | |
dc.date.accessioned | 2020-12-18T05:15:43Z | |
dc.identifier.issn | 0014-4835 | |
dc.identifier.uri | http://hdl.handle.net/1885/217412 | |
dc.description.abstract | Photobiomodulation (PBM) with 670 nm light has been shown to accelerate wound healing in soft tissue injuries, and also to protect neuronal tissues. However, little data exist on its effects on the non-neuronal components of the retina, such as Miller cells (MCs), which are the principal macroglia of the retina that play a role in maintaining retinal homeostasis. The aim of this study was to explore the effects of 670 nm light on activated MCs using in vivo and in 141 TO stress models. Adult Sprague-Dawley rats were exposed to photo-oxidative damage (PD) for 24 h and treated with 670 nm light at 0, 3 and 14 days after PD. Tissue was collected at 30 days post-PD for analysis. Using the in vitro scratch model with a human MC line (MIO-M1), area coverage and cellular stress were analysed following treatment with 670 nm light. We showed that early treatment with 670 nm light after PD reduced MC activation, lowering the retinal expression of GFAP and FGF-2. 670 nm light treatment mitigated the production of MC-related pro-inflammatory cytokines (including IL-1 beta), and reduced microglia/macrophage (MG/M Phi) recruitment into the outer retina following PD. This subsequently decreased photoreceptor loss, slowing the progression of retinal degeneration. In vitro, we showed that 670 nm light directly modulated MC activation, reducing rates of area coverage by suppressing cellular proliferation and spreading. This study indicates that 670 nm light treatment post-injury may have therapeutic benefit when administered shortly after retinal damage, and could be useful for retinal degenerations where MC gliosis is a feature of disease progression. | |
dc.description.sponsorship | This work was supported by the Australian Government National Health and Medical Research Council Grant (APP1049990), the Australian Government Research Training Program and the Gretel and Gordon Bootes Foundation. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_AU | |
dc.publisher | Academic Press | |
dc.rights | © 2018 Elsevier Ltd. | |
dc.source | Experimental Eye Research | |
dc.subject | Müller cell | |
dc.subject | Gliosis | |
dc.subject | Retinal degeneration | |
dc.subject | 670 nm light | |
dc.subject | Inflammation | |
dc.title | 670nm light treatment following retinal injury modulates Muller cell gliosis: Evidence from in vivo and in vitro stress models | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 169 | |
dc.date.issued | 2018 | |
local.identifier.absfor | 111303 - Vision Science | |
local.identifier.ariespublication | u5234101xPUB77 | |
local.publisher.url | https://www.elsevier.com/en-au | |
local.type.status | Published Version | |
local.contributor.affiliation | Lu, Yen-Zhen (Angel), College of Health and Medicine, ANU | |
local.contributor.affiliation | Fernando, Nilisha, College of Health and Medicine, ANU | |
local.contributor.affiliation | Natoli, Riccardo, College of Health and Medicine, ANU | |
local.contributor.affiliation | Madigan, Michele, University of Sydney | |
local.contributor.affiliation | Valter, Krisztina, College of Health and Medicine, ANU | |
local.description.embargo | 2099-12-31 | |
dc.relation | http://purl.org/au-research/grants/nhmrc/1049990 | |
local.bibliographicCitation.startpage | 1 | |
local.bibliographicCitation.lastpage | 12 | |
local.identifier.doi | 10.1016/j.exer.2018.01.011 | |
local.identifier.absseo | 920107 - Hearing, Vision, Speech and Their Disorders | |
dc.date.updated | 2023-03-12T07:16:12Z | |
local.identifier.scopusID | 2-s2.0-85042908618 | |
local.identifier.thomsonID | WOS:000430158400001 | |
Collections | ANU Research Publications |
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