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Cell-free expression of natively folded hydrophobins

Siddiquee, Rezwan; Choi, Samuel Sung-chan; Lam, Shirley Siuley; Wang, Patrick; Qi, Ruhu; Otting, Gottfried; Sunde, Margaret; Kwan, Ann H


Hydrophobins are a family of cysteine-rich proteins unique to filamentous fungi. The proteins are produced in a soluble form but self-assemble into organised amphipathic layers at hydrophilic:hydrophobic interfaces. These layers contribute to transitions between wet and dry environments, spore dispersal and attachment to surfaces for growth and infection. Hydrophobins are characterised by four disulphide bonds that are critical to their structure and function. Thus, obtaining correctly...[Show more]

CollectionsANU Research Publications
Date published: 2020
Type: Journal article
Source: Protein Expression and Purification
DOI: 10.1016/j.pep.2020.105591


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