The cytoskeletal motor proteins Dynein and MyoV direct apical transport of Crumbs
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Aguilar-Aragon, Mario; Fletcher, Georgina; Thompson, Barry
Description
Crumbs (Crb in Drosophila; CRB1-3 in mammals) is a transmembrane determinant of epithelial cell polarity and a regulator of Hippo signalling. Crb is normally localized to apical cell-cell contacts, just above adherens junctions, but how apical trafficking of Crb is regulated in epithelial cells remains unclear. We use the Drosophila follicular epithelium to demonstrate that polarized trafficking of Crb is mediated by transport along microtubules by the motor protein Dynein and along actin...[Show more]
dc.contributor.author | Aguilar-Aragon, Mario | |
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dc.contributor.author | Fletcher, Georgina | |
dc.contributor.author | Thompson, Barry | |
dc.date.accessioned | 2020-11-18T23:14:39Z | |
dc.date.available | 2020-11-18T23:14:39Z | |
dc.identifier.issn | 0012-1606 | |
dc.identifier.uri | http://hdl.handle.net/1885/216201 | |
dc.description.abstract | Crumbs (Crb in Drosophila; CRB1-3 in mammals) is a transmembrane determinant of epithelial cell polarity and a regulator of Hippo signalling. Crb is normally localized to apical cell-cell contacts, just above adherens junctions, but how apical trafficking of Crb is regulated in epithelial cells remains unclear. We use the Drosophila follicular epithelium to demonstrate that polarized trafficking of Crb is mediated by transport along microtubules by the motor protein Dynein and along actin filaments by the motor protein Myosin-V (MyoV). Blocking transport of Crb-containing vesicles by Dynein or MyoV leads to accumulation of Crb within Rab11 endosomes, rather than apical delivery. The final steps of Crb delivery and stabilisation at the plasma membrane requires the exocyst complex and three apical FERM domain proteins – Merlin, Moesin and Expanded – whose simultaneous loss disrupts apical localization of Crb. Accordingly, a knock-in deletion of the Crb FERMbinding motif (FBM) also impairs apical localization. Finally, overexpression of Crb challenges this system, creating a sensitized background to identify components involved in cytoskeletal polarization, apical membrane trafficking and stabilisation of Crb at the apical domain. | |
dc.description.sponsorship | Funded by the Francis Crick Institute and the Australian National University. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_AU | |
dc.publisher | Academic Press | |
dc.rights | © 2019 The Authors. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Developmental Biology | |
dc.title | The cytoskeletal motor proteins Dynein and MyoV direct apical transport of Crumbs | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 459 | |
dc.date.issued | 2020 | |
local.identifier.absfor | 111201 - Cancer Cell Biology | |
local.identifier.absfor | 060403 - Developmental Genetics (incl. Sex Determination) | |
local.identifier.absfor | 060103 - Cell Development, Proliferation and Death | |
local.identifier.ariespublication | a383154xPUB11218 | |
local.publisher.url | http://www.journals.elsevier.com/developmental-biology/ | |
local.type.status | Published Version | |
local.contributor.affiliation | Aguilar-Aragon, Mario, The Francis Crick Institute | |
local.contributor.affiliation | Fletcher, Georgina, The Francis Crick Institute | |
local.contributor.affiliation | Thompson, Barry, College of Health and Medicine, ANU | |
local.bibliographicCitation.issue | 2 | |
local.bibliographicCitation.startpage | 126 | |
local.bibliographicCitation.lastpage | 137 | |
local.identifier.doi | 10.1016/j.ydbio.2019.12.009 | |
local.identifier.absseo | 920102 - Cancer and Related Disorders | |
dc.date.updated | 2020-07-19T08:26:53Z | |
dcterms.accessRights | Open Access | |
dc.provenance | © 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | |
dc.rights.license | CC BY license | |
Collections | ANU Research Publications |
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