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Adherens junction remodelling during mitotic rounding of pseudostratified epithelial cells

Aguilar‐Aragon, Mario; Bonello, Teresa; Bell, Graham P.; Fletcher, Georgina; Thompson, Barry

Description

Epithelial cells undergo cortical rounding at the onset of mitosis to enable spindle orientation in the plane of the epithelium. In cuboidal epithelia in culture, the adherens junction protein Ecadherin recruits Pins/LGN/GPSM2 and Mud/NuMA to orient the mitotic spindle. In the pseudostratified columnar epithelial cells of Drosophila, septate junctions recruit Mud/NuMA to orient the spindle, while Pins/LGN/GPSM2 is surprisingly dispensable. We show that these pseudostratified epithelial cells...[Show more]

dc.contributor.authorAguilar‐Aragon, Mario
dc.contributor.authorBonello, Teresa
dc.contributor.authorBell, Graham P.
dc.contributor.authorFletcher, Georgina
dc.contributor.authorThompson, Barry
dc.date.accessioned2020-09-23T01:30:25Z
dc.date.available2020-09-23T01:30:25Z
dc.identifier.issn1469-221X
dc.identifier.urihttp://hdl.handle.net/1885/211368
dc.description.abstractEpithelial cells undergo cortical rounding at the onset of mitosis to enable spindle orientation in the plane of the epithelium. In cuboidal epithelia in culture, the adherens junction protein Ecadherin recruits Pins/LGN/GPSM2 and Mud/NuMA to orient the mitotic spindle. In the pseudostratified columnar epithelial cells of Drosophila, septate junctions recruit Mud/NuMA to orient the spindle, while Pins/LGN/GPSM2 is surprisingly dispensable. We show that these pseudostratified epithelial cells downregulate Ecadherin as they round up for mitosis. Preventing cortical rounding by inhibiting Rho-kinase-mediated actomyosin contractility blocks downregulation of E-cadherin during mitosis. Mitotic activation of Rho-kinase depends on the RhoGEF ECT2/Pebble and its binding partners RacGAP1/MgcRacGAP/CYK4/Tum and MKLP1/KIF23/ZEN4/ Pav. Cell cycle control of these Rho activators is mediated by the Aurora A and B kinases, which act redundantly during mitotic rounding. Thus, in Drosophila pseudostratified epithelia, disruption of adherens junctions during mitosis necessitates planar spindle orientation by septate junctions to maintain epithelial integrity.
dc.description.sponsorshipThis work was funded by the Francis Crick Institute (FC0010180), which is a joint initiative of Cancer Research UK (FC0010180), the Medical Research Council (FC001180), and Wellcome (FC001180). This work was also funded by The Australian National University
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherEMBO Press
dc.rights© 2020 The Authors
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceEMBO Reports
dc.titleAdherens junction remodelling during mitotic rounding of pseudostratified epithelial cells
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume21
dc.date.issued2020
local.identifier.absfor111201 - Cancer Cell Biology
local.identifier.absfor060103 - Cell Development, Proliferation and Death
local.identifier.absfor060403 - Developmental Genetics (incl. Sex Determination)
local.identifier.ariespublicationu6269649xPUB395
local.publisher.urlhttp://embor.embopress.org/
local.type.statusPublished Version
local.contributor.affiliationAguilar‐Aragon, Mario, Francis Crick Institute
local.contributor.affiliationBonello, Teresa, College of Health and Medicine, ANU
local.contributor.affiliationBell, Graham P., Francis Crick Institute
local.contributor.affiliationFletcher, Georgina, The Francis Crick Institute
local.contributor.affiliationThompson, Barry, College of Health and Medicine, ANU
local.bibliographicCitation.issue4
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage16
local.identifier.doi10.15252/embr.201949700
local.identifier.absseo920102 - Cancer and Related Disorders
dc.date.updated2020-06-23T00:57:52Z
local.identifier.scopusID2-s2.0-85079151205
dcterms.accessRightsOpen Access
dc.provenance© 2020 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.licenseCC BY 4.0 license
CollectionsANU Research Publications

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