Skip navigation
Skip navigation

Heparin-dependent aggregation of hen egg white lysozyme reveals two distinct mechanisms of amyloid fibrillation

Nitani, Ayame; Muta, Hiroya; Adachi, Masayuki; So, Masatomo; Sasahara, Kenji; Sakurai, Kazumasa; Chatani, Eric; Naoe, Kazumitsu; Ogi, Hirotsugu; Hall, Damien; Goto, Yuji


Heparin, a biopolymer possessing high negative charge density, is known to accelerate amyloid fibrillation by various proteins. Using hen egg white lysozyme, we studied the effects of heparin on protein aggregation at low pH, raised temperature, and applied ultrasonic irradiation, conditions under which amyloid fibrillation was promoted. Heparin exhibited complex bimodal concentration-dependent effects, either accelerating or inhibiting fibrillation at pH 2.0 and 60 °C. At concentrations lower...[Show more]

CollectionsANU Research Publications
Date published: 2017
Type: Journal article
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M117.813097
Access Rights: Open Access


File Description SizeFormat Image
01_Nitani_Heparin-dependent_aggregation_2017.pdf3.12 MBAdobe PDFThumbnail

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator