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Heparin-dependent aggregation of hen egg white lysozyme reveals two distinct mechanisms of amyloid fibrillation

Nitani, Ayame; Muta, Hiroya; Adachi, Masayuki; So, Masatomo; Sasahara, Kenji; Sakurai, Kazumasa; Chatani, Eric; Naoe, Kazumitsu; Ogi, Hirotsugu; Hall, Damien; Goto, Yuji

Description

Heparin, a biopolymer possessing high negative charge density, is known to accelerate amyloid fibrillation by various proteins. Using hen egg white lysozyme, we studied the effects of heparin on protein aggregation at low pH, raised temperature, and applied ultrasonic irradiation, conditions under which amyloid fibrillation was promoted. Heparin exhibited complex bimodal concentration-dependent effects, either accelerating or inhibiting fibrillation at pH 2.0 and 60 °C. At concentrations lower...[Show more]

CollectionsANU Research Publications
Date published: 2017
Type: Journal article
URI: http://hdl.handle.net/1885/210623
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M117.813097
Access Rights: Open Access

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