Heparin-dependent aggregation of hen egg white lysozyme reveals two distinct mechanisms of amyloid fibrillation
Heparin, a biopolymer possessing high negative charge density, is known to accelerate amyloid fibrillation by various proteins. Using hen egg white lysozyme, we studied the effects of heparin on protein aggregation at low pH, raised temperature, and applied ultrasonic irradiation, conditions under which amyloid fibrillation was promoted. Heparin exhibited complex bimodal concentration-dependent effects, either accelerating or inhibiting fibrillation at pH 2.0 and 60 °C. At concentrations lower...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of Biological Chemistry|
|Access Rights:||Open Access|
|01_Nitani_Heparin-dependent_aggregation_2017.pdf||3.12 MB||Adobe PDF|
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