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Detection and identification of Lactobacillus species in the crop of broilers of different ages using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis

Guan, Le Lou; Hagen, Karen; Tannock, G W; Korver, Doug; Fasenko, G M; Allison, Gwen


The microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA restriction analysis (ARDRA). The birds were raised under conditions similar to those used in commercial broiler production. Lactobacilli predominated and attained populations of 108 to 109 CFU per gram of crop contents. Many of the...[Show more]

CollectionsANU Research Publications
Date published: 2003
Type: Journal article
Source: Applied and Environmental Microbiology
DOI: 10.1128/AEM.69.11.6750-6757.2003


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