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MicroRNA MIMIC binding sites: Minor flanking nucleotide alterations can strongly impact MIMIC silencing efficacy in Arabidopsis

Wong, Gigi; Alonso-Peral, Maria; Li, Bingjun; Li, Junyan; Millar, Anthony

Description

In plants, microRNA (miRNA ) target MIMIC s (MIM s) have been widely used to inhibit miRNA function. They are based on the Arabidopsis INSENSITIVE TO PHOSPATE STARVATION 1 (IPS 1 ) gene that corresponds to a non‐coding RNA containing a miR399 binding site that can be modified to sequester and inhibit any miRNA of interest. However, the efficacy of miRNA inhibition of these different MIM s can vary greatly. Using MIM s that have strong efficacy (MIM 159 ) and poor efficacy (MIM 165 ), we...[Show more]

CollectionsANU Research Publications
Date published: 2018
Type: Journal article
URI: http://hdl.handle.net/1885/209994
Source: Plant Direct
DOI: 10.1002/pld3.88
Access Rights: Open Access

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