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Histone Acetylation, VERNALIZATION INSENSITIVE 3, FLOWERING LOCUS C, and the Vernalization Response

Bond, Donna; Dennis, Elizabeth; Pogson, Barry; Finnegan, E. Jean

Description

The quantitative induction of VIN3 by low temperatures is required for PRC2 repression of FLC and promotion of flowering (vernalization) in Arabidopsis. Histone acetylation, a chromatin modification commonly associated with gene transcription, increased on VIN3 chromatin in two spatially and temporally distinct phases in response to low temperatures. During short-term cold exposure, histone H3 acetylation at the transcription start site rapidly increased, implying that it is required for VIN3...[Show more]

dc.contributor.authorBond, Donna
dc.contributor.authorDennis, Elizabeth
dc.contributor.authorPogson, Barry
dc.contributor.authorFinnegan, E. Jean
dc.date.accessioned2015-12-07T22:23:55Z
dc.identifier.issn1752-9859
dc.identifier.urihttp://hdl.handle.net/1885/20934
dc.description.abstractThe quantitative induction of VIN3 by low temperatures is required for PRC2 repression of FLC and promotion of flowering (vernalization) in Arabidopsis. Histone acetylation, a chromatin modification commonly associated with gene transcription, increased on VIN3 chromatin in two spatially and temporally distinct phases in response to low temperatures. During short-term cold exposure, histone H3 acetylation at the transcription start site rapidly increased, implying that it is required for VIN3 induction. Subsequent changes in histone H3 and H4 acetylation occurred following continued VIN3 transcription during prolonged cold exposure. Members of the SAGA-like transcriptional adaptor complex, including the histone acetyltransferase GCN5, which induces expression of the cold acclimation pathway genes, do not regulate VIN3 induction during cold exposure, indicating that the cold acclimation pathway and the cold-induction of VIN3 are regulated by different transcriptional mechanisms. Mutations in the other 11 histone acetyltransferase genes did not affect VIN3 induction. However, nicotinamide, a histone deacetylase inhibitor, induced VIN3 and altered histone acetylation at the VIN3 locus. VIN3 induction was proportional to the length of nicotinamide treatment, which was associated with an early-flowering phenotype and repression of FLC. However, unlike vernalization, the repression of FLC was independent of VIN3 activity. Nicotinamide treatment did not cause a change in the expression of any genes in the autonomous pathway or members of the PRC2 complex, the well characterized repressors of FLC. Our data suggest that FLC is repressed via a novel pathway involving the SIR2 class of histone deacetylases.
dc.publisherOxford University Press
dc.sourceMolecular Plant
dc.subjectKeywords: Arabidopsis protein; FLF protein, Arabidopsis; histone; MADS domain protein; nicotinamide; acetylation; Arabidopsis; article; chromatin immunoprecipitation; cold; drug effect; gene expression regulation; genetics; metabolism; physiology; polymerase chain Cold acclimation; Histone acetyltransferase; Histone deacetylase; Nicotinamide; SIR2
dc.titleHistone Acetylation, VERNALIZATION INSENSITIVE 3, FLOWERING LOCUS C, and the Vernalization Response
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume2
dc.date.issued2009
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationu8611701xPUB14
local.type.statusPublished Version
local.contributor.affiliationBond, Donna, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationDennis, Elizabeth, CSIRO
local.contributor.affiliationPogson, Barry, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationFinnegan, E. Jean, CSIRO
local.description.embargo2037-12-31
local.bibliographicCitation.issue4
local.bibliographicCitation.startpage724
local.bibliographicCitation.lastpage737
local.identifier.doi10.1093/mp/ssp021
dc.date.updated2016-02-24T11:41:12Z
local.identifier.scopusID2-s2.0-68449104035
local.identifier.thomsonID000268331700016
CollectionsANU Research Publications

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