Small neutral Gd(iii) tags for distance measurements in proteins by double electron–electron resonance experiments
Spin labels containing a Gd(III) ion have become important for measuring nanometer distances in proteins by double electron–electron resonance (DEER) experiments at high EPR frequencies. The distance resolution and sensitivity of these measurements strongly depend on the Gd(III) tag used. Here we report the performance of two Gd(III) tags, propargyl-DO3A and C11 in DEER experiments carried out at W-band (95 GHz). Both tags are small, uncharged and devoid of bulky hydrophobic pendants. The...[Show more]
|Collections||ANU Research Publications|
|Source:||Physical Chemistry Chemical Physics|
|Access Rights:||Open Access|
|Small Neutral Gd(III) Tags.pdf||1.3 MB||Adobe PDF|
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