Using tert-Butyl Groups in a Ligand To Identify Its Binding Site on a Protein
Few methods allow determining the binding site of tightly binding ligands. We show that ligands containing a tert-butyl (e.g., Boc) group produce easily observable nuclear Overhauser effects (NOE) with the target protein even when the tert-butyl group is not highly solvent exposed. NOEs with methyl groups of the target protein are readily assigned by selectively isotope labeling, presenting a practical and quick way to pinpoint the location of the ligand without any prior specific nuclear...[Show more]
|Collections||ANU Research Publications|
|Source:||ACS medicinal chemistry letters|
|Access Rights:||Open Access|
|Using tert-Butyl Groups.pdf||394.44 kB||Adobe PDF|
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