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Limited Intravascular Coupling in the Rodent Brainstem and Retina Supports a Role for Glia in Regional Blood Flow

Kuo, Ivana; Chan-Ling, Tailoi; Wojcikiewicz, Richard J H; Hill, Caryl

Description

Regional synaptic activity induces local increases in perfusion that are coupled to upstream vasodilation and improved blood flow. In the cerebral circulation, it has been proposed that astrocytes mediate the link between the initiating stimulus and local vasodilation through propagated intracellular calcium waves. In the systemic circulation the mechanism by which local vasodilation triggers upstream alterations in blood flow involves electrotonic propagation of hyperpolarization via...[Show more]

dc.contributor.authorKuo, Ivana
dc.contributor.authorChan-Ling, Tailoi
dc.contributor.authorWojcikiewicz, Richard J H
dc.contributor.authorHill, Caryl
dc.date.accessioned2015-12-07T22:23:23Z
dc.identifier.issn0021-9967
dc.identifier.urihttp://hdl.handle.net/1885/20653
dc.description.abstractRegional synaptic activity induces local increases in perfusion that are coupled to upstream vasodilation and improved blood flow. In the cerebral circulation, it has been proposed that astrocytes mediate the link between the initiating stimulus and local vasodilation through propagated intracellular calcium waves. In the systemic circulation the mechanism by which local vasodilation triggers upstream alterations in blood flow involves electrotonic propagation of hyperpolarization via endothelial gap junctions, although less is known concerning the cerebral circulation. The present study aimed to investigate the extent of coupling in microvessels of the rodent brainstem and retina and the subtypes of intracellular calcium stores that might mediate astrocytic signaling. Within the brainstem, connexins (Cxs) 37 and 40 were restricted to the endothelium of pial vessels and larger penetrating arterioles, whereas astrocytic Cxs30 and 43 were found closely associated with pre- and postsynaptic neurons and nearby microvessels. Within the rat retina, Cxs37 and 40 were expressed in large radiating arterioles, but were not found in smaller vessels on the retinal surface or in the deeper retinal layers. These Cxs were absent from all retinal vessels in mice. Astrocytes, expressing Cxs30 and 43 in the rat, but only Cx43 in the mouse, were found closely associated with superficial, but not deeper blood vessels. Inositol-trisphosphate receptors (IP3R) 1 and 2 were expressed within brainstem astrocytes, whereas IP3R1 and 3 were expressed within retinal astrocytes. Limited intravascular coupling and the proximity of astrocytic networks to blood vessels supports a role for glia in activity-dependent alterations in central blood flow.
dc.publisherJohn Wiley & Sons Inc
dc.sourceJournal of Comparative Neurology
dc.subjectKeywords: connexin 37; connexin 40; connexin 43; glial fibrillary acidic protein; inositol 1,4,5 trisphosphate receptor; gap junction protein; animal cell; animal tissue; arteriole; article; astrocyte; auditory brainstem; brain blood flow; brain blood vessel; brain Astrocytes; Calcium stores; Connexins; Immunohistochemistry; IP3R
dc.titleLimited Intravascular Coupling in the Rodent Brainstem and Retina Supports a Role for Glia in Regional Blood Flow
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume511
dc.date.issued2008
local.identifier.absfor110901 - Autonomic Nervous System
local.identifier.ariespublicationu4693331xPUB13
local.type.statusPublished Version
local.contributor.affiliationKuo, Ivana, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationChan-Ling, Tailoi, University of Sydney
local.contributor.affiliationWojcikiewicz, Richard J H, State University of New York
local.contributor.affiliationHill, Caryl, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue6
local.bibliographicCitation.startpage773
local.bibliographicCitation.lastpage787
local.identifier.doi10.1002/cne.21873
dc.date.updated2016-02-24T11:17:46Z
local.identifier.scopusID2-s2.0-58749105837
local.identifier.thomsonID000260842300005
CollectionsANU Research Publications

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