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FadA-positive Fusobacterium nucleatum is prevalent in biopsy specimens of Iranian patients with colorectal cancer

Kashani, N.; Abadi, Amin Talebi Bezmin; Rahimi, Farid; Forootan, Mojgan

Description

Fusobacterium nucleatum has been increasingly implicated as a causative agent of various diseases, such as inflammatory bowel disease. Moreover, the gastrointestinal tracts of patients with colorectal cancer (CRC) also have been shown to be colonized by this bacterium. We aimed to determine the prevalence of F. nucleatum among CRC and non-CRC Iranian patients and to investigate potential associations between fadA-positive F. nucleatum and diagnosed CRC cases. Eighty patients admitted to two...[Show more]

dc.contributor.authorKashani, N.
dc.contributor.authorAbadi, Amin Talebi Bezmin
dc.contributor.authorRahimi, Farid
dc.contributor.authorForootan, Mojgan
dc.date.accessioned2020-07-17T02:00:42Z
dc.date.available2020-07-17T02:00:42Z
dc.identifier.issn2052-2975
dc.identifier.urihttp://hdl.handle.net/1885/206328
dc.description.abstractFusobacterium nucleatum has been increasingly implicated as a causative agent of various diseases, such as inflammatory bowel disease. Moreover, the gastrointestinal tracts of patients with colorectal cancer (CRC) also have been shown to be colonized by this bacterium. We aimed to determine the prevalence of F. nucleatum among CRC and non-CRC Iranian patients and to investigate potential associations between fadA-positive F. nucleatum and diagnosed CRC cases. Eighty patients admitted to two main hospitals in Tehran, Iran, were enrolled. The patients were aged between 20 and 75 and were diagnosed by a gastroenterologist. A trained surgeon used standard surgical protocols to collect two CRC biopsy samples per patient. One of the samples was used for pathologic examination, and the other was subjected to DNA extraction and PCR. Lesion colonization by F. nucleatum and expression of its major virulence factor, fadA, were investigated. The fadA-positive F. nucleatum strain was absent in all the lesions obtained from non-CRC patients. All patients with lesions that were colonized with fadA-positive F. nucleatum were diagnosed as CRC (p < 0.05); selected patients were sent for further intensive treatment. We found a significant association between the presence of F. nucleatum colonization and lesions from CRC patients (p 0.0001; odds ratio, 6.74; 95% confidence interval, 2.5-18.07). Our study confirmed colonization of the fadA-positive F. nucleatum on lesions from 80 Iranian CRC patients. New therapeutic strategies to achieve eradication of F. nucleatum are necessary for clinical management of patients suspected of having or prone to developing CRC.
dc.description.sponsorshipFinancially supported by the research deputy of Tarbiat Modares University, Tehran, Iran.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherElsevier Ltd
dc.rights© 2020 The Authors
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceNew Microbes and New Infections
dc.titleFadA-positive Fusobacterium nucleatum is prevalent in biopsy specimens of Iranian patients with colorectal cancer
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume34
dcterms.dateAccepted2020-01-03
dc.date.issued2020-01-09
local.identifier.absfor110310 - Intensive Care
local.identifier.absfor060501 - Bacteriology
local.identifier.absfor110307 - Gastroenterology and Hepatology
local.identifier.ariespublicationu6269649xPUB380
local.type.statusPublished Version
local.contributor.affiliationKashani, N., Tarbiat Modares University
local.contributor.affiliationAbadi, Amin Talebi Bezmin, Tarbiat Modares University
local.contributor.affiliationRahimi, Ahmed (Farid), College of Science, ANU
local.contributor.affiliationForootan, Mojgan, Shahid Beheshti University of Medical Sciences
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage5
local.identifier.doi10.1016/j.nmni.2020.100651
local.identifier.absseo920102 - Cancer and Related Disorders
local.identifier.absseo920109 - Infectious Diseases
local.identifier.absseo920105 - Digestive System Disorders
dc.date.updated2020-04-05T08:19:49Z
local.identifier.scopusID2-s2.0-85078772957
dcterms.accessRightsOpen Access
dc.provenanceThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.licenseCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
CollectionsANU Research Publications

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