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Localized two-photon photoswitching of Optovin in rat cortical neurons

Kheradpezhouh, Ehsan; Choy, Julian; Arabzadeh, Ehsan; Daria, Vincent

Description

Photoswitching molecules are widely used to control neuronal activity by activation of endogenous cellular channels and receptors. Optovin is a photoswitching molecule that targets the cation channel transient receptor potential ankyrin 1 (TRPA1). In zebrafish, bulk single-photon photoswitching of Optovin produced behavioural responses associated with TRPA1 activation. We recently found that TRPA1 is widely expressed in the rodent brain particularly in cortical pyramidal neurons and...[Show more]

dc.contributor.authorKheradpezhouh, Ehsan
dc.contributor.authorChoy, Julian
dc.contributor.authorArabzadeh, Ehsan
dc.contributor.authorDaria, Vincent
dc.date.accessioned2020-07-07T00:27:52Z
dc.identifier.citationEhsan Kheradpezhouh et al 2019 J. Phys. D: Appl. Phys. 52 254001
dc.identifier.issn0022-3727
dc.identifier.urihttp://hdl.handle.net/1885/205854
dc.description.abstractPhotoswitching molecules are widely used to control neuronal activity by activation of endogenous cellular channels and receptors. Optovin is a photoswitching molecule that targets the cation channel transient receptor potential ankyrin 1 (TRPA1). In zebrafish, bulk single-photon photoswitching of Optovin produced behavioural responses associated with TRPA1 activation. We recently found that TRPA1 is widely expressed in the rodent brain particularly in cortical pyramidal neurons and demonstrated its functional activation via TRPA1 agonists and by two-photon (2P) photoswitching of Optovin in the soma. Here, we exploit the highly localized 2P photoswitching of Optovin to activate TRPA1 along different dendritic and axonal regions of individual pyramidal neurons in the rodent cortex. The technique facilitates measurement of isolated responses when light is directed to the axon and along various dendritic domains of L5 pyramidal neurons. Using this technique, we show that 2P photoswitching of Optovin results in higher levels of depolarization along the base of the apical trunk compared to any other location in the neuron.
dc.description.sponsorshipThis work was supported by the Australian Research Council (ARC) Discovery Projects (DP130101364, DP170100908 and DP140101555), the National Health and Medical Research Council (NHMRC) Project Grants (PG1124411 and PG1105944) and the ARC Centre of Excellence for Integrative Brain Function (CE140100007).
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherInstitute of Physics Publishing
dc.rights© 2019 IOP Publishing Ltd
dc.sourceJournal of Physics D: Applied Physics
dc.subjecttwo-photon photoswitching
dc.subjectOptovin
dc.subjectcortical pyramidal neurons
dc.subjectTRPA1 channel
dc.titleLocalized two-photon photoswitching of Optovin in rat cortical neurons
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume52
dcterms.dateAccepted2019-03-25
dc.date.issued2019-04-12
local.identifier.absfor020503 - Nonlinear Optics and Spectroscopy
local.identifier.absfor060105 - Cell Neurochemistry
local.identifier.absfor110902 - Cellular Nervous System
local.identifier.ariespublicationu3102795xPUB1879
local.publisher.urlhttps://iopscience.iop.org/
local.type.statusPublished Version
local.contributor.affiliationKheradpezhouh, Ehsan, College of Health and Medicine, ANU
local.contributor.affiliationChoy, Julian, College of Health and Medicine, ANU
local.contributor.affiliationArabzadeh, Ehsan, College of Health and Medicine, ANU
local.contributor.affiliationDaria, Vincent, College of Health and Medicine, ANU
local.description.embargo2037-12-31
dc.relationhttp://purl.org/au-research/grants/arc/DP130101364
dc.relationhttp://purl.org/au-research/grants/arc/DP170100908
dc.relationhttp://purl.org/au-research/grants/arc/DP140101555
dc.relationhttp://purl.org/au-research/grants/nhmrc/1124411
dc.relationhttp://purl.org/au-research/grants/nhmrc/1105944
dc.relationhttp://purl.org/au-research/grants/arc/CE140100007
local.bibliographicCitation.issue25
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage7
local.identifier.doi10.1088/1361-6463/ab1333
dc.date.updated2020-07-06T08:20:50Z
local.identifier.scopusID2-s2.0-85067338419
local.identifier.thomsonID4.64454E+11
CollectionsANU Research Publications

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