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Cell Lysate as a Platform Technology for Biocatalytic Synthesis and Nucleic Acid Amplification

Loan, Thomas


The research presented in this Thesis focuses on the enzymatic synthesis of value-added products using cell lysates prepared from E. coli as low-cost biocatalysts. Naturally abundant endogenous enzymes are leveraged where possible and these are augmented with heterologous proteins, incorporated by recombinant overexpression, to create multi-enzymatic cascade reactions. A robust ATP regeneration system was developed, utilizing the endogenous acetate kinase (Ack) with acetyl phosphate....[Show more]

CollectionsRestricted Theses
Date published: 2020
Type: Thesis (PhD)
DOI: 10.25911/5f0edb8ed690d
Embargoed until: 2021-07-15


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Thomas Loan PhD Thesis 2020.pdfThesis Material24.43 MBAdobe PDF    Request a copy
Plasmid DNA sequences.zipSupporting Material150.26 kBUnknown    Request a copy

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