Cell Lysate as a Platform Technology for Biocatalytic Synthesis and Nucleic Acid Amplification
The research presented in this Thesis focuses on the enzymatic synthesis of value-added products using cell lysates prepared from E. coli as low-cost biocatalysts. Naturally abundant endogenous enzymes are leveraged where possible and these are augmented with heterologous proteins, incorporated by recombinant overexpression, to create multi-enzymatic cascade reactions. A robust ATP regeneration system was developed, utilizing the endogenous acetate kinase (Ack) with acetyl phosphate....[Show more]
|Collections||Open Access Theses|
|Thomas Loan PhD Thesis 2020.pdf||Thesis Material||24.43 MB||Adobe PDF|
|Plasmid DNA sequences.zip||Supporting Material||150.26 kB||Unknown|
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