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Paramagnetic labelling of proteins and oligonucleotides for NMR

Su, Xun-Cheng; Otting, Gottfried

Description

Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.

CollectionsANU Research Publications
Date published: 2010
Type: Journal article
URI: http://hdl.handle.net/1885/20311
Source: Journal of Biomolecular NMR
DOI: 10.1007/s10858-009-9331-1

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