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Suitability of recombinant lipase immobilised on functionalised magnetic nanoparticles for fish oil hydrolysis

Verma, Madan L; Rao, Nalam M.; Tsuzuki, Takuya; Barrow, Colin J; Puri, Munish

Description

Recombinant Bacillus subtilis lipase was immobilised on magnetic nanoparticles by a facile covalent method and applied to fish oil hydrolysis. High loading of enzyme to the functionalised nanoparticle was achieved with a protein binding efficiency of 95%. Structural changes of the confined enzyme on the surface of the nanoparticles was investigated using transmission electron microscopy and spectroscopic techniques (attenuated total reflectance-Fourier transform infrared and circular...[Show more]

CollectionsANU Research Publications
Date published: 2019
Type: Journal article
URI: http://hdl.handle.net/1885/202005
Source: Catalysts
DOI: 10.3390/catal9050420
Access Rights: Creative Commons Attribution (CC BY) license

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