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PacBio amplicon sequencing for metabarcoding of mixed DNA samples from lichen herbarium specimens

Gueidan, Cécile; Elix, John; McCarthy, Patrick M.; Roux, Claude; Mallen-Cooper, Max; Kantvilas, Gintaras


The detection and identification of species of fungi in the environment using molecular methods heavily depends on reliable reference sequence databases. However, these databases are largely incomplete in terms of taxon coverage, and a significant effort is required from herbaria and living fungal collections for the mass-barcoding of well-identified and well-curated fungal specimens or strains. Here, a PacBio amplicon sequencing approach is applied to recent lichen herbarium specimens for...[Show more]

CollectionsANU Research Publications
Date published: 2019-06-03
Type: Journal article
Source: MycoKeys
DOI: 10.3897/mycokeys.53.34761
Access Rights: Open Access


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