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In silico identification and characterization of mRNA-like noncoding transcripts in Medicago truncatula.

Wen, Jiayu; Parker, Brian; Weiller, Georg

Description

Accumulating evidence suggests that that non-coding RNAs (ncRNAs) play key roles in gene regulation and may form the basis of an inter-gene communication system. Many ncRNAs are synthesized similar to mRNAs and can be detected through screening of polyA-rich EST or cDNA libraries. We developed a computational pipeline to screen EST and genomic sequence data for those transcribed genes with limited protein coding potential and applied this pipeline to the model legume Medicago truncatula. This...[Show more]

dc.contributor.authorWen, Jiayu
dc.contributor.authorParker, Brian
dc.contributor.authorWeiller, Georg
dc.date.accessioned2015-12-07T22:21:26Z
dc.identifier.issn1386-6338
dc.identifier.urihttp://hdl.handle.net/1885/20044
dc.description.abstractAccumulating evidence suggests that that non-coding RNAs (ncRNAs) play key roles in gene regulation and may form the basis of an inter-gene communication system. Many ncRNAs are synthesized similar to mRNAs and can be detected through screening of polyA-rich EST or cDNA libraries. We developed a computational pipeline to screen EST and genomic sequence data for those transcribed genes with limited protein coding potential and applied this pipeline to the model legume Medicago truncatula. This process identified a set of 503 mRNA-like transcripts that appear not to encode proteins. Further computational analysis showed that many of these ncRNA candidates share structural similarities to known ncRNAs and that they clearly differ from protein coding genes and non-transcribed regions in their base and oligonucleotide compositions, as well as in aspects of secondary structure. By using a machine learning approach, we show that the distinctive ncRNA features presented in this study can be used to discriminate most ncRNAs and may thus be useful for improving ncRNA prediction. Computational analysis of EST isolation frequencies in various plant tissues showed that the expression levels and expression profiles of the putative ncRNAs and mRNAs differ - most interestingly, the putative ncRNAs are highly expressed relative to mRNAs in the root nodule tissue and conserved only in closely related plants. The work presented here constitutes the first large-scale prediction and characterization of ncRNAs in legumes, and provides a basis for further research on elucidating ncRNA function in legume genomics.
dc.publisherIOS Press
dc.sourceIn Silico Biology
dc.source.urihttp://www.bioinfo.de/isb/2007/07/0034/main.html
dc.subjectKeywords: DNA base; messenger RNA; messenger RNA like untranslated RNA; oligonucleotide; unclassified drug; untranslated RNA; amino acid sequence; article; barrel medic; controlled study; DNA base composition; expressed sequence tag; gene expression profiling; gene EST; Feature classification; McRNA; Medicago truncatula; Model legume; MRNA-like ncRNA; SVM
dc.titleIn silico identification and characterization of mRNA-like noncoding transcripts in Medicago truncatula.
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume7
dc.date.issued2007
local.identifier.absfor060705 - Plant Physiology
local.identifier.absfor060407 - Genome Structure and Regulation
local.identifier.ariespublicationu9009731xPUB10
local.type.statusPublished Version
local.contributor.affiliationWen, Jiayu, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationParker, Brian, College of Engineering and Computer Science, ANU
local.contributor.affiliationWeiller, Georg, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage0034 1
local.bibliographicCitation.lastpage22
dc.date.updated2015-12-07T08:57:44Z
local.identifier.scopusID2-s2.0-38949143289
CollectionsANU Research Publications

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