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Development of a Rapid in planta BioID System as a Probe for Plasma Membrane-Associated Immunity Proteins

Conlan, Brendon; Stoll, Thomas; Gorman, Jeffrey J; Saur, Isabel ML; Rathjen, John

Description

Plant pathogens secrete effector molecules that suppress the plant immune response to facilitate disease development. AvrPto is a well-studied effector from the phytopathogenic bacterium Pseudomonas syringae. Here we utilize an in planta proximity dependent biotin ligase labeling technique (BioID) in combination with AvrPto to identify proximal proteins that are potential immune system components. The labeling technique biotinylated proteins proximal to AvrPto at the plasma-membrane allowing...[Show more]

CollectionsANU Research Publications
Date published: 2018
Type: Journal article
URI: http://hdl.handle.net/1885/196765
Source: Frontiers in Plant Science
DOI: 10.3389/fpls.2018.01882
Access Rights: Open Access

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