Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar
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Gessler, Arthur; Dluzniewska , Paulina; Kopriva, Stanislav; Strnad , Miroslav; Novak, Ondrej; Dietrich , Henriette; Rennenberg, Heinz
Description
The present study shows for the first time the influence of exogenously applied amino acids and cytokinin on the physiological and molecular aspects of N metabolism in poplar trees. In a short-term feeding experiment, glutamine or trans-zeatin riboside (tZR) was added directly to the nutrient solution. NO 3- net uptake declined significantly in response to both treatments. Feeding with glutamine brought about an increase in concentrations of different amino compounds in the roots (glutamine,...[Show more]
dc.contributor.author | Gessler, Arthur | |
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dc.contributor.author | Dluzniewska , Paulina | |
dc.contributor.author | Kopriva, Stanislav | |
dc.contributor.author | Strnad , Miroslav | |
dc.contributor.author | Novak, Ondrej | |
dc.contributor.author | Dietrich , Henriette | |
dc.contributor.author | Rennenberg, Heinz | |
dc.date.accessioned | 2015-12-07T22:19:42Z | |
dc.identifier.issn | 0140-7791 | |
dc.identifier.uri | http://hdl.handle.net/1885/19466 | |
dc.description.abstract | The present study shows for the first time the influence of exogenously applied amino acids and cytokinin on the physiological and molecular aspects of N metabolism in poplar trees. In a short-term feeding experiment, glutamine or trans-zeatin riboside (tZR) was added directly to the nutrient solution. NO 3- net uptake declined significantly in response to both treatments. Feeding with glutamine brought about an increase in concentrations of different amino compounds in the roots (glutamine, glutamate, alanine, γ-amino butyric acid (GABA) and NH4+, which negatively correlated with the net NO3- uptake. The plants showed a reduction of cytosolic glutamine synthetase 1 (GS1) transcript level in the roots. In addition, glutamine feeding changed the root-to-shoot distribution on N assimilation in favour of the leaves and plant internal N cycling. tZR treatment resulted in expansion of zeatin-type (Z-type) cytokinins in the roots and increased nitrate reductase (NR)-mRNA level. The results indicate that both particular amino acids and active cytokinins are involved in the feedback regulation of N uptake and metabolism in poplar. We propose that inhibition of N uptake by cytokinins in poplar is more complex than that mediated by amino compounds, and other effectors are involved in this regulation. | |
dc.publisher | Blackwell Publishing Ltd | |
dc.source | Plant Cell and Environment | |
dc.subject | Keywords: cytokinin; glutamate ammonia ligase; glutamine; glutamine synthetase I; nitrate; nitrate reductase; nitrogen; amino acid; feeding; nitrogen; nutrient; physiology; RNA; root; tree; article; aspen; drug effect; metabolism; plant root; time; xylem; Cytokinin Amino acids; N cycling; Trans-zeatin riboside | |
dc.title | Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 29 | |
dc.date.issued | 2006 | |
local.identifier.absfor | 069999 - Biological Sciences not elsewhere classified | |
local.identifier.ariespublication | u4441299xPUB8 | |
local.type.status | Published Version | |
local.contributor.affiliation | Gessler, Arthur, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Dluzniewska , Paulina , University of Freiburg | |
local.contributor.affiliation | Kopriva, Stanislav, University of Freiburg | |
local.contributor.affiliation | Strnad , Miroslav , Palacky University | |
local.contributor.affiliation | Novak, Ondrej, Palacky University | |
local.contributor.affiliation | Dietrich , Henriette , University of Freiburg | |
local.contributor.affiliation | Rennenberg, Heinz, University of Freiburg | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 7 | |
local.bibliographicCitation.startpage | 1284 | |
local.bibliographicCitation.lastpage | 1297 | |
local.identifier.doi | 10.1111/j.1365-3040.2006.01507.x | |
dc.date.updated | 2015-12-07T08:40:05Z | |
local.identifier.scopusID | 2-s2.0-33744949616 | |
Collections | ANU Research Publications |
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