Skip navigation
Skip navigation

Simultaneous determination of Rubisco carboxylase and oxygenase kinetic parameters in Triticum aestivum and Zea mays using membrane inlet mass spectrometry

Cousins, A. B.; Ghannoum, Oula; von Caemmerer, Susanne; Badger, Murray

Description

The lack of complete Rubisco kinetic data for numerous species is partly because of the time consuming nature of the multiple methods needed to assay all of the Rubisco parameters. We have developed a membrane inlet mass spectrometer method that simultaneously determines the rate of Rubisco carboxylation (vc) and oxygenation (vo), and the CO2 and O2 concentrations. Using the collected data, the Michaels-Menten equations for vc and vo in response to changing CO2 and O2 concentrations were...[Show more]

dc.contributor.authorCousins, A. B.
dc.contributor.authorGhannoum, Oula
dc.contributor.authorvon Caemmerer, Susanne
dc.contributor.authorBadger, Murray
dc.date.accessioned2015-12-07T22:19:19Z
dc.identifier.issn0140-7791
dc.identifier.urihttp://hdl.handle.net/1885/19270
dc.description.abstractThe lack of complete Rubisco kinetic data for numerous species is partly because of the time consuming nature of the multiple methods needed to assay all of the Rubisco parameters. We have developed a membrane inlet mass spectrometer method that simultaneously determines the rate of Rubisco carboxylation (vc) and oxygenation (vo), and the CO2 and O2 concentrations. Using the collected data, the Michaels-Menten equations for vc and vo in response to changing CO2 and O2 concentrations were simultaneously solved for the CO2 (Kc) and O2 (Ko) constants, the maximum turnover rates of the enzyme for CO2 (kcatCO2) and O2 (kcatO2) and the specificity for CO2 relative to O2 (Sc/o). In the C4 species Zea mays Kc was higher but Ko was lower compared with the C3 species Triticum aestivum. The kcatCO2 was higher and the kcatO2 lower in Z. mays compared with T. aestivum and Sc/o was similar in the two species. The Vomax/Vcmax was lower in Z. mays and thus did not correlate with changes in Sc/o. In conclusion, this mass spectrometer system provides a means of simultaneously determining the important Rubisco kinetic parameters, Kc, Ko, kcatCO2,kcatO2 and Sc/o from the same set of assays.
dc.publisherBlackwell Publishing Ltd
dc.sourcePlant Cell and Environment
dc.subjectKeywords: carbon dioxide; oxygen; ribulosebisphosphate carboxylase; bioassay; carbon dioxide; enzyme activity; maize; mass spectrometry; photosynthesis; reaction kinetics; article; enzymology; maize; mass spectrometry; metabolism; methodology; wheat; Carbon Dioxide CO2; Enzyme kinetics; Photosynthesis
dc.titleSimultaneous determination of Rubisco carboxylase and oxygenase kinetic parameters in Triticum aestivum and Zea mays using membrane inlet mass spectrometry
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume33
dc.date.issued2010
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationu4956746xPUB7
local.type.statusPublished Version
local.contributor.affiliationCousins, A. B. , Washington State University
local.contributor.affiliationGhannoum, Oula, University of Western Sydney
local.contributor.affiliationvon Caemmerer, Susanne, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBadger, Murray, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage444
local.bibliographicCitation.lastpage452
local.identifier.doi10.1111/j.1365-3040.2009.02095.x
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T11:29:25Z
local.identifier.scopusID2-s2.0-77949418703
local.identifier.thomsonID000274412500012
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Cousins_Simultaneous_determination_of_2010.pdf364.96 kBAdobe PDFThumbnail


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator