Skip navigation
Skip navigation

4-1BBL coexpression enhances HIV-specific CD8 T cell memory ina poxvirus prime-boost vaccine

Harrison, Jodie; Bertram, Edward; Boyle, David B; Coupar, Barbara E H; Ranasinghe, Charani; Ramshaw, Ian

Description

We have constructed a recombinant fowlpox virus expressing HIV antigens and the costimulatory molecule 4-1BBL. When included in the boost, but not the prime of a poxvirus prime-boost strategy, 4-1BBL significantly enhanced the anti-HIV T cell response generated to this vaccination in BALB/c mice, as detected by ex vivo IFNγ ELISPOT responses, intracellular cytokine staining to HIV Gag antigens, and enumeration of Gag-reactive CD8 T cells. 4-1BBL however, is not capable of modulating the CD4 T...[Show more]

dc.contributor.authorHarrison, Jodie
dc.contributor.authorBertram, Edward
dc.contributor.authorBoyle, David B
dc.contributor.authorCoupar, Barbara E H
dc.contributor.authorRanasinghe, Charani
dc.contributor.authorRamshaw, Ian
dc.date.accessioned2015-12-07T22:18:54Z
dc.identifier.issn0264-410X
dc.identifier.urihttp://hdl.handle.net/1885/19053
dc.description.abstractWe have constructed a recombinant fowlpox virus expressing HIV antigens and the costimulatory molecule 4-1BBL. When included in the boost, but not the prime of a poxvirus prime-boost strategy, 4-1BBL significantly enhanced the anti-HIV T cell response generated to this vaccination in BALB/c mice, as detected by ex vivo IFNγ ELISPOT responses, intracellular cytokine staining to HIV Gag antigens, and enumeration of Gag-reactive CD8 T cells. 4-1BBL however, is not capable of modulating the CD4 T cell response, nor the antibody response to this vaccination strategy. Enhancement of the T cell response by 4-1BBL continues into the memory phase, as detected 2 months post vaccination. This data is the first to show modulation of the immune response to a viral vaccine by coexpression of 4-1BBL and supports this strategy as an exciting approach for enhancement of T cell memory in prime-boost vaccines.
dc.publisherElsevier
dc.sourceVaccine
dc.subjectKeywords: poxvirus prime boost vaccine; unclassified drug; virus vaccine; animal cell; animal experiment; animal model; animal tissue; antibody response; antigen expression; article; cell stimulation; controlled study; cytokine release; enzyme linked immunospot ass Costimulation; T cells; Vaccination
dc.title4-1BBL coexpression enhances HIV-specific CD8 T cell memory ina poxvirus prime-boost vaccine
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume24
dc.date.issued2006
local.identifier.absfor110704 - Cellular Immunology
local.identifier.ariespublicationu6800332xPUB6
local.type.statusPublished Version
local.contributor.affiliationHarrison, Jodie, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBertram, Edward, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBoyle, David B, CSIRO Division of Livestock Industries
local.contributor.affiliationCoupar, Barbara E H, Duke University
local.contributor.affiliationRanasinghe, Charani, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRamshaw, Ian, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage6867
local.bibliographicCitation.lastpage6874
local.identifier.doi10.1016/j.vaccine.2006.06.007
dc.date.updated2015-12-07T08:28:24Z
local.identifier.scopusID2-s2.0-33846070524
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Harrison_4-1BBL_coexpression_enhances_2006.pdf542.37 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator