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Development and validation of a multiplexed-tandem qPCR tool for diagnostics of human soil-transmitted helminth infections

Stracke, Katharina; Clarke, Naomi; Awburn, Camille; Vaz Nery, Susana; Khieu, Virak; Traub, Rebecca; Jex, Aaron Richard

Description

Soil-transmitted helminths (STH) are a major cause of morbidity in tropical developing countries with a global infection prevalence of more than one billion people and disease burden of around 3.4 million disability adjusted life years. Infection prevalence directly correlates to inadequate sanitation, impoverished conditions and limited access to public health systems. Underestimation of infection prevalence using traditional microscopy-based diagnostic techniques is common, specifically in...[Show more]

dc.contributor.authorStracke, Katharina
dc.contributor.authorClarke, Naomi
dc.contributor.authorAwburn, Camille
dc.contributor.authorVaz Nery, Susana
dc.contributor.authorKhieu, Virak
dc.contributor.authorTraub, Rebecca
dc.contributor.authorJex, Aaron Richard
dc.date.accessioned2019-12-09T00:58:12Z
dc.date.available2019-12-09T00:58:12Z
dc.identifier.citationStracke K, Clarke N, Awburn CV, Vaz Nery S, Khieu V, Traub RJ, et al. (2019) Development and validation of a multiplexed-tandem qPCR tool for diagnostics of human soil-transmitted helminth infections. PLoS Negl Trop Dis 13(6): e0007363. https://doi.org/10.1371/journal.pntd.0007363
dc.identifier.issn1935-2735
dc.identifier.urihttp://hdl.handle.net/1885/188488
dc.description.abstractSoil-transmitted helminths (STH) are a major cause of morbidity in tropical developing countries with a global infection prevalence of more than one billion people and disease burden of around 3.4 million disability adjusted life years. Infection prevalence directly correlates to inadequate sanitation, impoverished conditions and limited access to public health systems. Underestimation of infection prevalence using traditional microscopy-based diagnostic techniques is common, specifically in populations with access to benzimidazole mass treatment programs and a predominance of low intensity infections. In this study, we developed a multiplexed-tandem qPCR (MT-PCR) tool to identify and quantify STH eggs in stool samples. We have assessed this assay by measuring infection prevalence and intensity in field samples of two cohorts of participants from Timor-Leste and Cambodia, which were collected as part of earlier epidemiological studies. MT-PCR diagnostic parameters were compared to a previously published multiplexed qPCR for STH detection. The MT-PCR assay agreed strongly with qPCR data and showed a diagnostic specificity of 99.60–100.00% (sensitivity of 83.33–100.00%) compared to qPCR and kappa agreement exceeding 0.85 in all tests. In addition, the MT-PCR has the added advantage of distinguishing Ancylostoma spp. species, namely Ancylostoma duodenale and Ancylostoma ceylanicum. This semi-automated platform uses a standardized, manufactured reagent kit, shows excellent run-to-run consistency/repeatability and supports high-throughput detection and quantitation at a moderate cost.
dc.description.sponsorshipThis work, including the efforts of ARJ, was funded by a NHMRC Career Development Fellowship (APP1126395). KS, CVA, RJT and ARJ are supported by the Victorian State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme. KS is supported by a Melbourne Research Scholarship (Australian Government) and the Riady Scholarship (Victoria, Australia).
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherPublic Library of Science
dc.rights© 2019 Stracke et al.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLoS Neglected Tropical Diseases
dc.subjectDiagnostic medicine
dc.subjectHelminth infections
dc.subjectNecator americanus
dc.subjectTrichuriasis
dc.subjectAncylostoma
dc.subjectPolymerase chain reaction
dc.subjectAscaris lumbricoides
dc.titleDevelopment and validation of a multiplexed-tandem qPCR tool for diagnostics of human soil-transmitted helminth infections
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume13
dcterms.dateAccepted2019-04-05
dc.date.issued2019-06-17
local.identifier.absfor110309 - Infectious Diseases
local.identifier.absfor110803 - Medical Parasitology
local.identifier.absfor111706 - Epidemiology
local.identifier.ariespublicationu1067127xPUB53
local.publisher.urlhttps://journals.plos.org/
local.type.statusPublished Version
local.contributor.affiliationStracke Katharina, The Walter and Eliza Hall Institute of Medical Research
local.contributor.affiliationClarke, Naomi, College of Health and Medicine, ANU
local.contributor.affiliationAwburn, Camille, The Walter and Eliza Hall Institute of Medical Research
local.contributor.affiliationVaz Nery, Susana, College of Health and Medicine, ANU
local.contributor.affiliationKhieu, Virak, Ministry of Health, Phnom Penh
local.contributor.affiliationTraub, Rebecca, University of Melbourne
local.contributor.affiliationJex, Aaron Richard, The University of Melbourne
dc.relationhttp://purl.org/au-research/grants/nhmrc/1126395
local.bibliographicCitation.issue6
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage14
local.identifier.doi10.1371/journal.pntd.0007363
local.identifier.absseo920404 - Disease Distribution and Transmission (incl. Surveillance and Response)
local.identifier.absseo920503 - Health Related to Specific Ethnic Groups
local.identifier.absseo920206 - Health Inequalities
dc.date.updated2019-07-28T08:17:28Z
dcterms.accessRightsOpen Access
dc.provenanceThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.licenseCreative Commons Attribution License
CollectionsANU Research Publications

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