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Harnessing the MinION: An example of how to establish long-read sequencing in a laboratory using challenging plant tissue from Eucalyptus pauciflora

Schalamun, Miriam; Nagar, Ramawater; Kainer, David; Beavan, Eleanor; Eccles, David; Rathjen, John; Lanfear, Robert; Schwessinger, Benjamin

Description

Long‐read sequencing technologies are transforming our ability to assemble highly complex genomes. Realizing their full potential is critically reliant on extracting high‐quality, high‐molecular‐weight (HMW) DNA from the organisms of interest. This is especially the case for the portable MinION sequencer which enables all laboratories to undertake their own genome sequencing projects, due to its low entry cost and minimal spatial footprint. One challenge of the MinION is that each group has to...[Show more]

CollectionsANU Research Publications
Date published: 2019-01
Type: Journal article
URI: http://hdl.handle.net/1885/186341
Source: Molecular Ecology Resources
DOI: 10.1111/1755-0998.12938
Access Rights: Open Access

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