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NMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction

chen, weihua; Salari, Hooman; Taylor, Matthew C.; Jost, Ricarda; Berkowitz, Oliver; Barrow, Russell; qiu, deyun; Branco, Remi; Masle, Josette

Description

Phosphatidylcholine (PC) is a major membrane phospholipid and a precursor for major signaling molecules. Understanding its synthesis is important for improving plant growth, nutritional value, and resistance to stress. PC synthesis is complex, involving several interconnected pathways, one of which proceeds from serine-derived phosphoethanolamine to form phosphocholine through three sequential phospho-base methylations catalyzed by phosphoethanolamine N-methyltransferases (PEAMTs). The...[Show more]

dc.contributor.authorchen, weihua
dc.contributor.authorSalari, Hooman
dc.contributor.authorTaylor, Matthew C.
dc.contributor.authorJost, Ricarda
dc.contributor.authorBerkowitz, Oliver
dc.contributor.authorBarrow, Russell
dc.contributor.authorqiu, deyun
dc.contributor.authorBranco, Remi
dc.contributor.authorMasle, Josette
dc.date.accessioned2019-11-18T01:26:16Z
dc.identifier.issn1532-2548
dc.identifier.urihttp://hdl.handle.net/1885/186129
dc.description.abstractPhosphatidylcholine (PC) is a major membrane phospholipid and a precursor for major signaling molecules. Understanding its synthesis is important for improving plant growth, nutritional value, and resistance to stress. PC synthesis is complex, involving several interconnected pathways, one of which proceeds from serine-derived phosphoethanolamine to form phosphocholine through three sequential phospho-base methylations catalyzed by phosphoethanolamine N-methyltransferases (PEAMTs). The contribution of this pathway to the production of PC and plant growth has been a matter of some debate. Although a handful of individual PEAMTs have been described, there has not been any in planta investigation of a PEAMT family. Here, we provide a comparative functional analysis of two Arabidopsis (Arabidopsis thaliana) PEAMTs, NMT1 and the little known NMT3. Analysis of loss-of-function mutants demonstrates that NMT1 and NMT3 synergistically regulate PC homeostasis, phase transition at the shoot apex, coordinated organ development, and fertility through overlapping but also specific functions. The nmt1 nmt3 double mutant shows extensive sterility, drastically reduced PC concentrations, and altered lipid profiles. These findings demonstrate that the phospho-base methylation pathway makes a major contribution to PC synthesis in Arabidopsis and that NMT1 and NMT3 play major roles in its catalysis and the regulation of PC homeostasis as well as in plant growth and reproduction.
dc.format.extent24 pages
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherAmerican Society of Plant Biologists
dc.rights© 2018 American Society of Plant Biologists
dc.sourcePlant Physiology
dc.titleNMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume177
dcterms.dateAccepted2018-05-10
dc.date.issued2018-08
local.identifier.absfor060703 - Plant Developmental and Reproductive Biology
local.identifier.absfor060702 - Plant Cell and Molecular Biology
local.identifier.ariespublicationu4485658xPUB1780
local.publisher.urlhttp://www.aspbjournals.org/
local.type.statusPublished Version
local.contributor.affiliationChen, Weihua, College of Science, The Australian National University
local.contributor.affiliationSalari, Hooman, Razi University
local.contributor.affiliationTaylor, Matthew C., Commonwealth Scientific and Industrial Research Organisation Land and Water Flagship
local.contributor.affiliationJost, Ricarda, College of Science, The Australian National University
local.contributor.affiliationBerkowitz, Oliver, College of Science, The Australian National University
local.contributor.affiliationBarrow, Russell, College of Science, The Australian National University
local.contributor.affiliationQiu, Deyun, College of Science, The Australian National University
local.contributor.affiliationBranco, Remi, College of Science, The Australian National University
local.contributor.affiliationMasle, Josette , College of Science, The Australian National University
local.description.embargo2037-12-31
local.bibliographicCitation.startpage1605
local.bibliographicCitation.lastpage1628
local.identifier.doi10.1104/pp.18.00457
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2019-05-05T09:24:11Z
local.identifier.scopusID2-s2.0-85059698324
local.identifier.thomsonID000440980500022
CollectionsANU Research Publications

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