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Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA

Squires, Jeffrey; Patel, Hardip; Nousch, Marco; Sibbritt, Tennille; Humphreys, David T; Parker, Brian J; Suter, Catherine M; Preiss, Thomas


The modified base 5-methylcytosine (m 5C) is well studied in DNA, but investigations of its prevalence in cellular RNA have been largely confined to tRNA and rRNA. In animals, the two m 5C methyltransferases NSUN2 and TRDMT1 are known to modify specific tRNAs and have roles in the control of cell growth and differentiation. To map modified cytosine sites across a human transcriptome, we coupled bisulfite conversion of cellular RNA with next-generation sequencing. We confirmed 21 of the 28...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
Source: Nucleic Acids Research
DOI: 10.1093/nar/gks144
Access Rights: Open Access


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