Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA
The modified base 5-methylcytosine (m 5C) is well studied in DNA, but investigations of its prevalence in cellular RNA have been largely confined to tRNA and rRNA. In animals, the two m 5C methyltransferases NSUN2 and TRDMT1 are known to modify specific tRNAs and have roles in the control of cell growth and differentiation. To map modified cytosine sites across a human transcriptome, we coupled bisulfite conversion of cellular RNA with next-generation sequencing. We confirmed 21 of the 28...[Show more]
|Collections||ANU Research Publications|
|Source:||Nucleic Acids Research|
|01_Squires_Widespread_occurrence_of_2012.pdf||551.31 kB||Adobe PDF||Request a copy|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.