Developmental kinetics, turnover and stimulatory capacity of thymic epithelial cells
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Gray, Daniel H D; Seach, Natalie; Ueno, Tomoo; Milton, Morag; Liston, Adrian; Lew, Andrew M; Goodnow, Christopher; Boyd, Richard L
Description
Despite the importance of thymic stromal cells to T-cell development, relatively little is known about their biology. Here, we use single-cell analysis of stromal cells to analyze extensive changes in the number and composition of thymic stroma throughout life, revealing a surprisingly dynamic population. Phenotypic progression of thymic epithelial subsets was assessed at high resolution in young mice to provide a developmental framework. The cellular and molecular requirements of adult...[Show more]
dc.contributor.author | Gray, Daniel H D | |
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dc.contributor.author | Seach, Natalie | |
dc.contributor.author | Ueno, Tomoo | |
dc.contributor.author | Milton, Morag | |
dc.contributor.author | Liston, Adrian | |
dc.contributor.author | Lew, Andrew M | |
dc.contributor.author | Goodnow, Christopher | |
dc.contributor.author | Boyd, Richard L | |
dc.date.accessioned | 2015-12-07T22:17:20Z | |
dc.identifier.issn | 0006-4971 | |
dc.identifier.uri | http://hdl.handle.net/1885/18495 | |
dc.description.abstract | Despite the importance of thymic stromal cells to T-cell development, relatively little is known about their biology. Here, we use single-cell analysis of stromal cells to analyze extensive changes in the number and composition of thymic stroma throughout life, revealing a surprisingly dynamic population. Phenotypic progression of thymic epithelial subsets was assessed at high resolution in young mice to provide a developmental framework. The cellular and molecular requirements of adult epithelium were studied, using various mutant mice to demonstrate new cross talk checkpoints dependent on RelB in the cortex and CD40 in the medulla. With the use of Ki67 and BrdU labeling, the turnover of thymic epithelium was found to be rapid, but then diminished on thymic involution. The various defects in stromal turnover and composition that accompanied involution were rapidly reversed following sex steroid ablation. Unexpectedly, mature cortical and medullary epithelium showed a potent capacity to stimulate naive T cells, comparable to that of thymic dendritic cells. Overall, these studies show that the thymic stroma is a surprisingly dynamic population and may have a more direct role in negative selection than previously thought. | |
dc.publisher | American Society of Hematology | |
dc.source | Blood | |
dc.subject | Keywords: broxuridine; CD40 antigen; CD45 antigen; Ki 67 antigen; sex hormone; transcription factor RelB; animal cell; article; autoimmunity; cell count; cell function; cell kinetics; cell maturation; cell population; cell proliferation; cellular immunity; controll | |
dc.title | Developmental kinetics, turnover and stimulatory capacity of thymic epithelial cells | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 108 | |
dc.date.issued | 2006 | |
local.identifier.absfor | 110704 - Cellular Immunology | |
local.identifier.ariespublication | u6800332xPUB4 | |
local.type.status | Published Version | |
local.contributor.affiliation | Gray, Daniel H D, Monash University | |
local.contributor.affiliation | Seach, Natalie, Monash University | |
local.contributor.affiliation | Ueno, Tomoo, Monash University | |
local.contributor.affiliation | Milton, Morag, Monash University | |
local.contributor.affiliation | Liston, Adrian, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Lew, Andrew M, Walter and Eliza Hall Institute of Medical Research | |
local.contributor.affiliation | Goodnow, Christopher, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Boyd, Richard L, Monash University | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 12 | |
local.bibliographicCitation.startpage | 3777 | |
local.bibliographicCitation.lastpage | 3785 | |
local.identifier.doi | 10.1182/blood-2006-02-004531 | |
dc.date.updated | 2015-12-07T08:05:23Z | |
local.identifier.scopusID | 2-s2.0-33845239216 | |
Collections | ANU Research Publications |
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