Structural biology by NMR spectroscopy
Pseudocontact shifts (PCSs) generated by a paramagnetic metal ion provide valuable long-range information about the protein structure. Tagging a protein with a paramagnetic metal centre is commonly achieved using cysteine ligation. This thesis mainly focuses on using a new technique of attaching a paramagnetic metal onto the proteins which is better immobilized and does not impact the protein structure. Following a general introduction in Chapter 1, Chapter 2 explores the binding of...[Show more]
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