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Magnetic quantitative reverse transcription PCR: A high-throughput method for mRNA extraction and quantitative reverse transcription PCR

Jost, Ricarda; Berkowitz, Oliver; Masle, Josette


Over the past few years high-throughput platforms for real-time quantitative PCR have become widely available. The cost of RNA extraction from a large number of samples are, however, quite notable. One method that stands out with respect to free up- or downscaling of sample size and reliability is the isolation of mRNA using oligodeoxythymidylate [oligo(dT)25]-coated magnetic particles. In combining this magnetic separation of mRNA with real-time reverse transcription PCR (RT-PCR), we have...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: BioTechniques
DOI: 10.2144/000112534


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