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Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Eom, Jennifer; Feisst, Vaughan; Ranjard, Louis; Loomes, Kerry; Damani, Tanvi; Jackson-Patel, Victoria; Locke, Michelle; Sheppard, Hilary; Narayan, Pritika; Dunbar, P. Rod


Several dyes are currently available for use in detecting differentiation of mesenchymal cells into adipocytes. Dyes, such as Oil Red O, are cheap, easy to use and widely utilized by laboratories analyzing the adipogenic potential of mesenchymal cells. However, they are not specific to changes in gene transcription. We have developed a gene-specific differentiation assay to analyze when a mesenchymal cell has switched its fate to an adipogenic lineage. Immuno-labelling against fatty acid...[Show more]

CollectionsANU Research Publications
Date published: 2018-03-31
Type: Journal article
Source: Journal of Visualized Experiments
DOI: 10.3791/57153
Access Rights: Open Access


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