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Hyperthermophilic Carbamate Kinase Stability and Anabolic In Vitro Activity at Alkaline pH

Hennessy, James E; Latter, Melissa; Philbrook, Amy; Bartkus, Daniel; Kim, Hye; Onagi, Hideki; Oakeshott, J.G.; Scott, Colin; Alissandratos, Apostolos; Easton, Christopher

Description

Carbamate kinases catalyze the conversion of carbamate to carbamoyl phosphate, which is readily transformed into other compounds. Carbamate forms spontaneously from ammonia and carbon dioxide in aqueous solutions, so the kinases have potential for sequestrative utilization of the latter compounds. Here, we compare seven carbamate kinases from mesophilic, thermophilic, and hyperthermophilic sources. In addition to the known enzymes from Enterococcus faecalis and Pyrococcus furiosus, the...[Show more]

CollectionsANU Research Publications
Date published: 2018
Type: Journal article
URI: http://hdl.handle.net/1885/171673
Source: Applied and Environmental Microbiology
DOI: 10.1128/AEM.02250-17

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