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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Arita, Yoshihiko; Torres-Mapa, Maria Leilani; Lee, Woei Ming; Čižmár, Tomáš; Campbell, Paul; Gunn-Moore, Frank J.; Dholakia, Kishan

Description

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite...[Show more]

dc.contributor.authorArita, Yoshihiko
dc.contributor.authorTorres-Mapa, Maria Leilani
dc.contributor.authorLee, Woei Ming
dc.contributor.authorČižmár, Tomáš
dc.contributor.authorCampbell, Paul
dc.contributor.authorGunn-Moore, Frank J.
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2015-12-07T01:41:56Z
dc.date.available2015-12-07T01:41:56Z
dc.identifier.issn0003-6951
dc.identifier.urihttp://hdl.handle.net/1885/17053
dc.description.abstractWe demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
dc.description.sponsorshipWe thank the UK EPSRC for funding. K.D. is a Royal Society Wolfson-Merit Award Holder. P.C. acknowledges the MRC for a Discipline Hopping Fellowship and Milstein Award. M.L.T.-M. acknowledges the support of a SUPA Prize Studentship.
dc.publisherAmerican Institute of Physics (AIP)
dc.rightshttp://www.sherpa.ac.uk/romeo/issn/0003-6951..."Publishers version/PDF may be used on author's personal website, institutional website or institutional repository" from SHERPA/RoMEO site (as at 7/12/15). Copyright 2011 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Applied Physics Letters and may be found at https://doi.org/10.1063/1.3554415
dc.sourceApplied Physics Letters
dc.subjectKeywords: Cell lysis; DNA transfection; Gene transfection; Laser induced breakdown; Live cell; Multi-site; Nanosecond laser pulse; Nanosurgery; Parallelizing; Plasma membranes; Reduced energy; Spatial light modulators; Trapped nanoparticles; Cell membranes; Genes;
dc.titleSpatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume98
dc.date.issued2011-03-02
local.identifier.absfor090699
local.identifier.absfor091299
local.identifier.absfor020599
local.identifier.ariespublicationu4628727xPUB133
local.publisher.urlhttps://www.aip.org/
local.type.statusPublished Version
local.contributor.affiliationArita, Yoshihiko, University of St Andrews, United Kingdom
local.contributor.affiliationTorres-Mapa, Maria Leilani, University of St Andrews, United Kingdom
local.contributor.affiliationLee, Woei Ming (Steve), College of Engineering and Computer Science, College of Engineering and Computer Science, Research School of Engineering, The Australian National University
local.contributor.affiliationČižmár, Tomáš, University of St Andrews, United Kingdom
local.contributor.affiliationCampbell, Paul, University of Dundee, United Kingdom
local.contributor.affiliationGunn-Moore, Frank J., University of St Andrews, United Kingdom
local.contributor.affiliationDholakia, K, University of St Andrews, United Kingdom
local.bibliographicCitation.issue9
local.bibliographicCitation.startpage093702
local.bibliographicCitation.lastpage4
local.identifier.doi10.1063/1.3554415
local.identifier.absseo970109
local.identifier.absseo970110
local.identifier.absseo970102
dc.date.updated2016-02-24T11:14:36Z
local.identifier.scopusID2-s2.0-79952407617
CollectionsANU Research Publications

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