Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
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Arita, Yoshihiko; Torres-Mapa, Maria Leilani; Lee, Woei Ming; Čižmár, Tomáš; Campbell, Paul; Gunn-Moore, Frank J.; Dholakia, Kishan
Description
We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite...[Show more]
dc.contributor.author | Arita, Yoshihiko | |
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dc.contributor.author | Torres-Mapa, Maria Leilani | |
dc.contributor.author | Lee, Woei Ming | |
dc.contributor.author | Čižmár, Tomáš | |
dc.contributor.author | Campbell, Paul | |
dc.contributor.author | Gunn-Moore, Frank J. | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2015-12-07T01:41:56Z | |
dc.date.available | 2015-12-07T01:41:56Z | |
dc.identifier.issn | 0003-6951 | |
dc.identifier.uri | http://hdl.handle.net/1885/17053 | |
dc.description.abstract | We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique. | |
dc.description.sponsorship | We thank the UK EPSRC for funding. K.D. is a Royal Society Wolfson-Merit Award Holder. P.C. acknowledges the MRC for a Discipline Hopping Fellowship and Milstein Award. M.L.T.-M. acknowledges the support of a SUPA Prize Studentship. | |
dc.publisher | American Institute of Physics (AIP) | |
dc.rights | http://www.sherpa.ac.uk/romeo/issn/0003-6951..."Publishers version/PDF may be used on author's personal website, institutional website or institutional repository" from SHERPA/RoMEO site (as at 7/12/15). Copyright 2011 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Applied Physics Letters and may be found at https://doi.org/10.1063/1.3554415 | |
dc.source | Applied Physics Letters | |
dc.subject | Keywords: Cell lysis; DNA transfection; Gene transfection; Laser induced breakdown; Live cell; Multi-site; Nanosecond laser pulse; Nanosurgery; Parallelizing; Plasma membranes; Reduced energy; Spatial light modulators; Trapped nanoparticles; Cell membranes; Genes; | |
dc.title | Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 98 | |
dc.date.issued | 2011-03-02 | |
local.identifier.absfor | 090699 | |
local.identifier.absfor | 091299 | |
local.identifier.absfor | 020599 | |
local.identifier.ariespublication | u4628727xPUB133 | |
local.publisher.url | https://www.aip.org/ | |
local.type.status | Published Version | |
local.contributor.affiliation | Arita, Yoshihiko, University of St Andrews, United Kingdom | |
local.contributor.affiliation | Torres-Mapa, Maria Leilani, University of St Andrews, United Kingdom | |
local.contributor.affiliation | Lee, Woei Ming (Steve), College of Engineering and Computer Science, College of Engineering and Computer Science, Research School of Engineering, The Australian National University | |
local.contributor.affiliation | Čižmár, Tomáš, University of St Andrews, United Kingdom | |
local.contributor.affiliation | Campbell, Paul, University of Dundee, United Kingdom | |
local.contributor.affiliation | Gunn-Moore, Frank J., University of St Andrews, United Kingdom | |
local.contributor.affiliation | Dholakia, K, University of St Andrews, United Kingdom | |
local.bibliographicCitation.issue | 9 | |
local.bibliographicCitation.startpage | 093702 | |
local.bibliographicCitation.lastpage | 4 | |
local.identifier.doi | 10.1063/1.3554415 | |
local.identifier.absseo | 970109 | |
local.identifier.absseo | 970110 | |
local.identifier.absseo | 970102 | |
dc.date.updated | 2016-02-24T11:14:36Z | |
local.identifier.scopusID | 2-s2.0-79952407617 | |
Collections | ANU Research Publications |
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