Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite...[Show more]
|Collections||ANU Research Publications|
|Source:||Applied Physics Letters|
|01_Arita_Spatially_optimized_gene_2011.pdf||Published Version||687.75 kB||Adobe PDF|
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