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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Arita, Yoshihiko; Torres-Mapa, Maria Leilani; Lee, Woei Ming; Čižmár, Tomáš; Campbell, Paul; Gunn-Moore, Frank J.; Dholakia, Kishan

Description

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite...[Show more]

CollectionsANU Research Publications
Date published: 2011-03-02
Type: Journal article
URI: http://hdl.handle.net/1885/17053
Source: Applied Physics Letters
DOI: 10.1063/1.3554415

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