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Arbitrary multi-site two-photon excitation in four dimensions

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Daria, Vincent Ricardo; Stricker, Christian; Bowman, Richard; Redman, Stephen; Bachor, Hans

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We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved...[Show more]

dc.contributor.authorDaria, Vincent Ricardo
dc.contributor.authorStricker, Christian
dc.contributor.authorBowman, Richard
dc.contributor.authorRedman, Stephen
dc.contributor.authorBachor, Hans
dc.date.accessioned2015-12-04T03:29:28Z
dc.date.available2015-12-04T03:29:28Z
dc.identifier.issn0003-6951
dc.identifier.urihttp://hdl.handle.net/1885/17010
dc.description.abstractWe demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.
dc.description.sponsorshipThis project has been funded by the Australian National University, Office of the Vice-chancellor and the major equipment grant 08MEC06. The work of H.A.B. was supported by the Australian Research Council Centre of Excellence scheme.
dc.publisherAmerican Institute of Physics (AIP)
dc.rightshttp://www.sherpa.ac.uk/romeo/issn/0003-6951..."Publishers version/PDF may be used on author's personal website, institutional website or institutional repository" from SHERPA/RoMEO site (as at 4/12/15). Copyright 2009 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Applied Physics Letters and may be found at https://doi.org/10.1063/1.3216581
dc.sourceApplied Physics Letters
dc.subjectKeywords: Biological cells; Graphics accelerators; High-speed; Holographic projection; Multi-site; Neuronal networks; Non-iterative; Optical Storage; Photon densities; Rapid response; Three dimensions; Time-resolved; Two-photon excitations; Volume holographic; Phot
dc.titleArbitrary multi-site two-photon excitation in four dimensions
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume95
dc.date.issued2009-09-02
local.identifier.absfor020503
local.identifier.absfor110999
local.identifier.ariespublicationu4492652xPUB2
local.publisher.urlhttps://www.aip.org/
local.type.statusPublished Version
local.contributor.affiliationDaria, Vincent, College of Physical and Mathematical Sciences, CPMS Research School of Physics and Engineering, Department of Quantum Science, The Australian National University
local.contributor.affiliationStricker, Christian, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Eccles Institute of Neuroscience, The Australian National University
local.contributor.affiliationBowman, Richard, University of Glasgow, United Kingdom
local.contributor.affiliationRedman, Stephen, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Eccles Institute of Neuroscience, The Australian National University
local.contributor.affiliationBachor, Hans, College of Physical and Mathematical Sciences, CPMS Research School of Physics and Engineering, Department of Quantum Science, The Australian National University
local.bibliographicCitation.issue9
local.bibliographicCitation.startpage093701
local.identifier.doi10.1063/1.3216581
dc.date.updated2016-02-24T11:11:01Z
local.identifier.scopusID2-s2.0-69949120443
local.identifier.thomsonID000269625800075
CollectionsANU Research Publications

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