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Comparative Pathogenomics Reveals Horizontally Acquired Novel Virulence Genes in Fungi Infecting Cereal Hosts

Gardiner, Donald M.; McDonald, Megan C.; Covarelli, Lorenzo; Solomon, Peter S.; Rusu, Anca G.; Marshall, Mhairi; Kazan, Kemal; Chakraborty, Sukumar; McDonald, Bruce A.; Manners, John M.

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Comparative analyses of pathogen genomes provide new insights into how pathogens have evolved common and divergent virulence strategies to invade related plant species. Fusarium crown and root rots are important diseases of wheat and barley world-wide. In Australia, these diseases are primarily caused by the fungal pathogen Fusarium pseudograminearum. Comparative genomic analyses showed that the F. pseudograminearum genome encodes proteins that are present in other fungal pathogens of cereals...[Show more]

dc.contributor.authorGardiner, Donald M.
dc.contributor.authorMcDonald, Megan C.
dc.contributor.authorCovarelli, Lorenzo
dc.contributor.authorSolomon, Peter S.
dc.contributor.authorRusu, Anca G.
dc.contributor.authorMarshall, Mhairi
dc.contributor.authorKazan, Kemal
dc.contributor.authorChakraborty, Sukumar
dc.contributor.authorMcDonald, Bruce A.
dc.contributor.authorManners, John M.
dc.date.accessioned2015-11-26T22:43:26Z
dc.date.available2015-11-26T22:43:26Z
dc.identifier.issn1553-7374
dc.identifier.urihttp://hdl.handle.net/1885/16854
dc.description.abstractComparative analyses of pathogen genomes provide new insights into how pathogens have evolved common and divergent virulence strategies to invade related plant species. Fusarium crown and root rots are important diseases of wheat and barley world-wide. In Australia, these diseases are primarily caused by the fungal pathogen Fusarium pseudograminearum. Comparative genomic analyses showed that the F. pseudograminearum genome encodes proteins that are present in other fungal pathogens of cereals but absent in non-cereal pathogens. In some cases, these cereal pathogen specific genes were also found in bacteria associated with plants. Phylogenetic analysis of selected F. pseudograminearum genes supported the hypothesis of horizontal gene transfer into diverse cereal pathogens. Two horizontally acquired genes with no previously known role in fungal pathogenesis were studied functionally via gene knockout methods and shown to significantly affect virulence of F. pseudograminearum on the cereal hosts wheat and barley. Our results indicate using comparative genomics to identify genes specific to pathogens of related hosts reveals novel virulence genes and illustrates the importance of horizontal gene transfer in the evolution of plant infecting fungal pathogens.
dc.description.sponsorshipDMG was supported by an Australian Research Council Post Doctoral Fellowship (www.arc.gov.au). The CSIRO Transformational Biology Capability Platform (www.csiro.au) supported the genome sequencing work presented in this manuscript. LC was supported by an Australian Government Endeavour foundation award (www.deewr.gov.au/International/EndeavourAwards/Pages/Home.aspx). MCM was supported by the ETH Zurich (www.ethz.ch). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.publisherPublic Library of Science
dc.rights© 2012 Gardiner et al.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLoS Pathogens
dc.subjectbase sequence
dc.subjectfungal proteins
dc.subjectfusarium
dc.subjectgene transfer, horizontal
dc.subjecthordeum
dc.subjectmolecular sequence data
dc.subjectphylogeny
dc.subjectplant diseases
dc.subjectsequence analysis, dna
dc.subjecttriticum
dc.subjectgenome, fungal
dc.titleComparative Pathogenomics Reveals Horizontally Acquired Novel Virulence Genes in Fungi Infecting Cereal Hosts
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume8
dc.date.issued2012-09-27
local.identifier.absfor060307
local.identifier.absfor060704
local.identifier.absfor060409
local.identifier.ariespublicationf5625xPUB1392
local.type.statusPublished Version
local.contributor.affiliationGardiner, Donald M , Commonwealth Scientific and Industrial Research Organization, Australia
local.contributor.affiliationMcDonald, Megan, ETH Zurich, Switzerland
local.contributor.affiliationCovarelli, Lorenzo, Commonwealth Scientific and Industrial Research Organization, Australia
local.contributor.affiliationSolomon, Peter, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Plant Sciences, The Australian National University
local.contributor.affiliationRusu, Anca G, Commonwealth Scientific and Industrial Research Organization, Australia
local.contributor.affiliationMarshall, Mhairi, University of Queensland, Australia
local.contributor.affiliationKazan, Kemal, CSIRO Division of Plant Industry, Australia
local.contributor.affiliationChakraborty, Sukumar, Commonwealth Scientific and Industrial Research Organization (CSIRO), Australia
local.contributor.affiliationMcDonald, Bruce A, Swiss Federal Institute of Technology (ETH), Switzerland
local.contributor.affiliationManners, John, CSIRO, Australia
local.identifier.essn1553-7374
local.bibliographicCitation.issue9
local.bibliographicCitation.startpagee1002952
local.identifier.doi10.1371/journal.ppat.1002952
local.identifier.absseo820507
local.identifier.absseo970106
local.identifier.absseo970107
dc.date.updated2016-02-24T08:46:06Z
local.identifier.scopusID2-s2.0-84866897256
local.identifier.thomsonID000309816500044
dcterms.accessRightsOpen Access
dc.rights.licenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
CollectionsANU Research Publications

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