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Establishment of an In Vitro System Representing the Chicken Gut-Associated Lymphoid Tissue

Alitheen, Noorjahan Banu; McClure, Susan Jane; Yeap, Swee Keong; Kristeen-Teo, Ye Wen; Tan, Sheau Wei; McCullagh, Peter

Description

The bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid...[Show more]

dc.contributor.authorAlitheen, Noorjahan Banu
dc.contributor.authorMcClure, Susan Jane
dc.contributor.authorYeap, Swee Keong
dc.contributor.authorKristeen-Teo, Ye Wen
dc.contributor.authorTan, Sheau Wei
dc.contributor.authorMcCullagh, Peter
dc.date.accessioned2015-11-26T05:23:02Z
dc.date.available2015-11-26T05:23:02Z
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1885/16842
dc.description.abstractThe bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid by well organized epithelium. Whereas lymphocytes within agglomerates were predominantly Bu-1a(+), a majority of those emigrating onto the supporting membrane were Bu-1a(-) and IgM(+). Both agglomerates and emigrant cells expressed activation-induced deaminase with levels increasing after 24 hours. Emigrating cells were actively proliferating at a rate in excess of both the starting cell population and the population of cells remaining in agglomerates. The potential usefulness of this system for investigating the response of bursal tissue to avian Newcastle disease virus (strain AF2240) was examined.
dc.description.sponsorshipThis study was supported by a Fundamental grant (project no. 05-13-03-040J) from the Ministry of Higher Education, Malaysia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.publisherPublic Library of Science
dc.rights© 2012 Alitheen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.sourcePLoS ONE
dc.source.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0049188
dc.subjectanimals
dc.subjectb-lymphocytes
dc.subjectbursa of fabricius
dc.subjectcell aggregation
dc.subjectcell membrane
dc.subjectcell movement
dc.subjectcell proliferation
dc.subjectcells, cultured
dc.subjectchick embryo
dc.subjectchickens
dc.subjectcytidine deaminase
dc.subjectepithelial cells
dc.subjectepithelium
dc.subjectfluoresceins
dc.subjectfluorescence
dc.subjectgastrointestinal tract
dc.subjectki-67 antigen
dc.subjectnewcastle disease
dc.subjectnewcastle disease virus
dc.subjectperoxidase
dc.subjectphenotype
dc.subjectreal-time polymerase chain reaction
dc.subjectspleen
dc.subjectstaining and labeling
dc.subjectsuccinimides
dc.titleEstablishment of an In Vitro System Representing the Chicken Gut-Associated Lymphoid Tissue
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume7
dc.date.issued2012-11-19
local.identifier.absfor110799
local.identifier.ariespublicationf5625xPUB1999
local.publisher.urlhttp://journals.plos.org/
local.type.statusPublished Version
local.contributor.affiliationAlitheen, Noorjahan Banu, University Putra Malaysia, Malaysia
local.contributor.affiliationMcClure, Susan J, CSIRO Division of Livestock Industries, Australia
local.contributor.affiliationYeap, Swee Keong, University Putra Malaysia, Malaysia
local.contributor.affiliationKristeen-Teo, Ye Wen, University Putra Malaysia, Malaysia
local.contributor.affiliationTan, Sheau Wei, University Putra Malaysia, Malaysia
local.contributor.affiliationMcCullagh, Peter, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Immunology and Infectious Disease, The Australian National University
local.identifier.essn1932-6203
local.bibliographicCitation.issue11
local.bibliographicCitation.startpagee49188
local.identifier.doi10.1371/journal.pone.0049188
dc.date.updated2015-12-10T11:31:35Z
local.identifier.scopusID2-s2.0-84869775441
local.identifier.thomsonID000312394400003
CollectionsANU Research Publications

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