Establishment of an In Vitro System Representing the Chicken Gut-Associated Lymphoid Tissue
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Alitheen, Noorjahan Banu; McClure, Susan Jane; Yeap, Swee Keong; Kristeen-Teo, Ye Wen; Tan, Sheau Wei; McCullagh, Peter
Description
The bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid...[Show more]
dc.contributor.author | Alitheen, Noorjahan Banu | |
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dc.contributor.author | McClure, Susan Jane | |
dc.contributor.author | Yeap, Swee Keong | |
dc.contributor.author | Kristeen-Teo, Ye Wen | |
dc.contributor.author | Tan, Sheau Wei | |
dc.contributor.author | McCullagh, Peter | |
dc.date.accessioned | 2015-11-26T05:23:02Z | |
dc.date.available | 2015-11-26T05:23:02Z | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/1885/16842 | |
dc.description.abstract | The bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid by well organized epithelium. Whereas lymphocytes within agglomerates were predominantly Bu-1a(+), a majority of those emigrating onto the supporting membrane were Bu-1a(-) and IgM(+). Both agglomerates and emigrant cells expressed activation-induced deaminase with levels increasing after 24 hours. Emigrating cells were actively proliferating at a rate in excess of both the starting cell population and the population of cells remaining in agglomerates. The potential usefulness of this system for investigating the response of bursal tissue to avian Newcastle disease virus (strain AF2240) was examined. | |
dc.description.sponsorship | This study was supported by a Fundamental grant (project no. 05-13-03-040J) from the Ministry of Higher Education, Malaysia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | |
dc.publisher | Public Library of Science | |
dc.rights | © 2012 Alitheen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.source | PLoS ONE | |
dc.source.uri | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0049188 | |
dc.subject | animals | |
dc.subject | b-lymphocytes | |
dc.subject | bursa of fabricius | |
dc.subject | cell aggregation | |
dc.subject | cell membrane | |
dc.subject | cell movement | |
dc.subject | cell proliferation | |
dc.subject | cells, cultured | |
dc.subject | chick embryo | |
dc.subject | chickens | |
dc.subject | cytidine deaminase | |
dc.subject | epithelial cells | |
dc.subject | epithelium | |
dc.subject | fluoresceins | |
dc.subject | fluorescence | |
dc.subject | gastrointestinal tract | |
dc.subject | ki-67 antigen | |
dc.subject | newcastle disease | |
dc.subject | newcastle disease virus | |
dc.subject | peroxidase | |
dc.subject | phenotype | |
dc.subject | real-time polymerase chain reaction | |
dc.subject | spleen | |
dc.subject | staining and labeling | |
dc.subject | succinimides | |
dc.title | Establishment of an In Vitro System Representing the Chicken Gut-Associated Lymphoid Tissue | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 7 | |
dc.date.issued | 2012-11-19 | |
local.identifier.absfor | 110799 | |
local.identifier.ariespublication | f5625xPUB1999 | |
local.publisher.url | http://journals.plos.org/ | |
local.type.status | Published Version | |
local.contributor.affiliation | Alitheen, Noorjahan Banu, University Putra Malaysia, Malaysia | |
local.contributor.affiliation | McClure, Susan J, CSIRO Division of Livestock Industries, Australia | |
local.contributor.affiliation | Yeap, Swee Keong, University Putra Malaysia, Malaysia | |
local.contributor.affiliation | Kristeen-Teo, Ye Wen, University Putra Malaysia, Malaysia | |
local.contributor.affiliation | Tan, Sheau Wei, University Putra Malaysia, Malaysia | |
local.contributor.affiliation | McCullagh, Peter, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Immunology and Infectious Disease, The Australian National University | |
local.identifier.essn | 1932-6203 | |
local.bibliographicCitation.issue | 11 | |
local.bibliographicCitation.startpage | e49188 | |
local.identifier.doi | 10.1371/journal.pone.0049188 | |
dc.date.updated | 2015-12-10T11:31:35Z | |
local.identifier.scopusID | 2-s2.0-84869775441 | |
local.identifier.thomsonID | 000312394400003 | |
Collections | ANU Research Publications |
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