Skip navigation
Skip navigation

Strategies for wheat stripe rust pathogenicity identified by transcriptome sequencing

Garnica, Diana P; Upadhyaya, Narayana M; Dodds, Peter N; Rathjen, John P

Description

Stripe rust caused by the fungus Puccinia striiformis f.sp. tritici (Pst) is a major constraint to wheat production worldwide. The molecular events that underlie Pst pathogenicity are largely unknown. Like all rusts, Pst creates a specialized cellular structure within host cells called the haustorium to obtain nutrients from wheat, and to secrete pathogenicity factors called effector proteins. We purified Pst haustoria and used next-generation sequencing platforms to assemble the haustorial...[Show more]

dc.contributor.authorGarnica, Diana P
dc.contributor.authorUpadhyaya, Narayana M
dc.contributor.authorDodds, Peter N
dc.contributor.authorRathjen, John P
dc.date.accessioned2015-11-25T03:23:33Z
dc.date.available2015-11-25T03:23:33Z
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1885/16744
dc.description.abstractStripe rust caused by the fungus Puccinia striiformis f.sp. tritici (Pst) is a major constraint to wheat production worldwide. The molecular events that underlie Pst pathogenicity are largely unknown. Like all rusts, Pst creates a specialized cellular structure within host cells called the haustorium to obtain nutrients from wheat, and to secrete pathogenicity factors called effector proteins. We purified Pst haustoria and used next-generation sequencing platforms to assemble the haustorial transcriptome as well as the transcriptome of germinated spores. 12,282 transcripts were assembled from 454-pyrosequencing data and used as reference for digital gene expression analysis to compare the germinated uredinospores and haustoria transcriptomes based on Illumina RNAseq data. More than 400 genes encoding secreted proteins which constitute candidate effectors were identified from the haustorial transcriptome, with two thirds of these up-regulated in this tissue compared to germinated spores. RT-PCR analysis confirmed the expression patterns of 94 effector candidates. The analysis also revealed that spores rely mainly on stored energy reserves for growth and development, while haustoria take up host nutrients for massive energy production for biosynthetic pathways and the ultimate production of spores. Together, these studies substantially increase our knowledge of potential Pst effectors and provide new insights into the pathogenic strategies of this important organism.
dc.description.sponsorshipJ.P.R. is an ARC Future Fellow (FT0992129). This project has been supported by Bioplatforms Australia through funding from the Commonwealth Government NCRIS and Education Investment Fund Super Science programs.
dc.format17 pages
dc.publisherPublic Library of Science
dc.rights© 2013 Garnica et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.sourcePLoS ONE
dc.subjectstripe rust
dc.subjectPuccinia striiformis f.sp. tritici (Pst)
dc.subjectwheat production
dc.subjecthaustorium
dc.subjectpathogenicity factors
dc.subjecteffector proteins
dc.subjectnext-generation sequencing platforms
dc.subjecthaustorial transcriptome
dc.subjecttranscriptome of germinated spores
dc.titleStrategies for wheat stripe rust pathogenicity identified by transcriptome sequencing
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume8
dcterms.dateAccepted2013-05-14
dc.date.issued2013-06-26
local.identifier.absfor060102
local.identifier.absfor060307
local.identifier.absfor060704
local.identifier.ariespublicationf5625xPUB3577
local.publisher.urlhttps://www.plos.org/
local.type.statusPublished Version
local.contributor.affiliationGarnica, Diana, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Plant Sciences, The Australian National University
local.contributor.affiliationUpadhyaya, Narayana M, CSIRO Plant Industry, Australia
local.contributor.affiliationDodds, Peter N, CSIRO , Australia
local.contributor.affiliationRathjen, John, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Plant Sciences, The Australian National University
dc.relationhttp://purl.org/au-research/grants/arc/FT0992129
local.identifier.essn1932-6203
local.bibliographicCitation.issue6
local.bibliographicCitation.startpagee67150
local.identifier.doi10.1371/journal.pone.0067150
local.identifier.absseo970106
dc.date.updated2015-12-11T08:14:23Z
local.identifier.scopusID2-s2.0-84879497159
local.identifier.thomsonID000321424400075
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Garnica_Strategies_for_wheat_stripe_2013.pdfPublished Version2.92 MBAdobe PDFThumbnail


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  22 January 2019/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator