VHA-19 is essential in Caenorhabditis elegans oocytes for embryogenesis and is involved in trafficking in oocytes
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Knight, Alison J.; Johnson, Nicholas M.; Behm, Carolyn A.
Description
There is an urgent need to develop new drugs against parasitic nematodes, which are a significant burden on human health and agriculture. Information about the function of essential nematode-specific genes provides insight to key nematode-specific processes that could be targeted with drugs. We have characterized the function of a novel, nematode-specific Caenorhabditis elegans protein, VHA-19, and show that VHA-19 is essential in the germline and, specifically, the oocytes, for the completion...[Show more]
dc.contributor.author | Knight, Alison J. | |
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dc.contributor.author | Johnson, Nicholas M. | |
dc.contributor.author | Behm, Carolyn A. | |
dc.date.accessioned | 2015-11-23T22:42:08Z | |
dc.date.available | 2015-11-23T22:42:08Z | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/1885/16625 | |
dc.description.abstract | There is an urgent need to develop new drugs against parasitic nematodes, which are a significant burden on human health and agriculture. Information about the function of essential nematode-specific genes provides insight to key nematode-specific processes that could be targeted with drugs. We have characterized the function of a novel, nematode-specific Caenorhabditis elegans protein, VHA-19, and show that VHA-19 is essential in the germline and, specifically, the oocytes, for the completion of embryogenesis. VHA-19 is also involved in trafficking the oocyte receptor RME-2 to the oocyte plasma membrane and is essential for osmoregulation in the embryo, probably because VHA-19 is required for proper eggshell formation via exocytosis of cortical granules or other essential components of the eggshell. VHA-19 may also have a role in cytokinesis, either directly or as an indirect effect of its role in osmoregulation. Critically, VHA-19 is expressed in the excretory cell in both larvae and adults, suggesting that it may have a role in osmoregulation in C. elegans more generally, probably in trafficking or secretion pathways. This is the first time a role for VHA-19 has been described. | |
dc.description.sponsorship | AK was supported in part by a Junior Research Fellowship from Meat and Livestock Australia and by an Australian Government Postgraduate Award. | |
dc.format | 13 pages | |
dc.publisher | Public Library of Science | |
dc.rights | © 2012 Knight et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.source | PLoS ONE | |
dc.subject | animals | |
dc.subject | biological transport | |
dc.subject | caenorhabditis elegans | |
dc.subject | caenorhabditis elegans proteins | |
dc.subject | cell membrane | |
dc.subject | embryo, nonmammalian | |
dc.subject | embryonic development | |
dc.subject | humans | |
dc.subject | oocytes | |
dc.subject | water-electrolyte balance | |
dc.title | VHA-19 is essential in Caenorhabditis elegans oocytes for embryogenesis and is involved in trafficking in oocytes | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES. | |
local.identifier.citationvolume | 7 | |
dcterms.dateAccepted | 2012-06-04 | |
dc.date.issued | 2012-07-02 | |
local.identifier.absfor | 060400 | |
local.identifier.absfor | 060405 | |
local.identifier.ariespublication | f5625xPUB776 | |
local.publisher.url | https://www.plos.org/ | |
local.type.status | Published Version | |
local.contributor.affiliation | Knight, Alison, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Biomedical Science and Biochemistry, The Australian National University | |
local.contributor.affiliation | Johnson, Nick, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Biomedical Science and Biochemistry, The Australian National University | |
local.contributor.affiliation | Behm, Carolyn, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Biomedical Science and Biochemistry, The Australian National University | |
local.identifier.essn | 1932-6203 | |
local.bibliographicCitation.issue | 7 | |
local.bibliographicCitation.startpage | e40317 | |
local.identifier.doi | 10.1371/journal.pone.0040317 | |
dc.date.updated | 2015-12-10T09:06:11Z | |
local.identifier.scopusID | 2-s2.0-84863633054 | |
local.identifier.thomsonID | 000305966500064 | |
Collections | ANU Research Publications |
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