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Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C4 grasses

Danila, Florence; Quick, W Paul; White, Rosemary Gillian; Kelly, Steven; von Caemmerer, Susanne; Furbank, Robert

Description

Proliferation of plasmodesmata (PD) connections between bundle sheath (BS) and mesophyll (M) cells has been proposed as a key step in the evolution of two-cell C4 photosynthesis; However, a lack of quantitative data has hampered further exploration and validation of this hypothesis. In this study, we quantified leaf anatomical traits associated with metabolite transport in 18 species of BEP and PACMAD grasses encompassing four origins of C4 photosynthesis and all three C4 subtypes (NADP-ME,...[Show more]

dc.contributor.authorDanila, Florence
dc.contributor.authorQuick, W Paul
dc.contributor.authorWhite, Rosemary Gillian
dc.contributor.authorKelly, Steven
dc.contributor.authorvon Caemmerer, Susanne
dc.contributor.authorFurbank, Robert
dc.date.accessioned2019-07-31T23:30:46Z
dc.date.available2019-07-31T23:30:46Z
dc.identifier.issn0022-0957
dc.identifier.urihttp://hdl.handle.net/1885/164869
dc.description.abstractProliferation of plasmodesmata (PD) connections between bundle sheath (BS) and mesophyll (M) cells has been proposed as a key step in the evolution of two-cell C4 photosynthesis; However, a lack of quantitative data has hampered further exploration and validation of this hypothesis. In this study, we quantified leaf anatomical traits associated with metabolite transport in 18 species of BEP and PACMAD grasses encompassing four origins of C4 photosynthesis and all three C4 subtypes (NADP-ME, NAD-ME, and PCK). We demonstrate that C4 leaves have greater PD density between M and BS cells than C3 leaves. We show that this greater PD density is achieved by increasing either the pit field (cluster of PD) area or the number of PD per pit field area. NAD-ME species had greater pit field area per M–BS interface than NADP-ME or PCK species. In contrast, NADP-ME and PCK species had lower pit field area with increased number of PD per pit field area than NAD-ME species. Overall, PD density per M–BS cell interface was greatest in NAD-ME species while PD density in PCK species exhibited the largest variability. Finally, the only other anatomical characteristic that clearly distinguished C4 from C3 species was their greater Sb value, the BS surface area to subtending leaf area ratio. In contrast, BS cell volume was comparable between the C3 and C4 grass species examined.
dc.description.sponsorshipFRD is supported by scholarship awards from the Lee Foundation (IRRI) and the Australian Research Council Centre of Excellence for Translational Photosynthesis (CE140100015). SK is a Royal Society University Research Fellow. Work in SK’s lab is supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement number 637765.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherOxford University Press
dc.rights© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceJournal of Experimental Botany
dc.titleMultiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C4 grasses
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume69
dc.date.issued2018
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationu4956746xPUB719
local.publisher.urlhttp://www.oxfordjournals.org/
local.type.statusPublished Version
local.contributor.affiliationDanila , Florence, College of Science, ANU
local.contributor.affiliationQuick, W Paul, University of Sheffield
local.contributor.affiliationWhite, Rosemary Gillian, CSIRO Division of Plant Industry
local.contributor.affiliationKelly, Steven, University of Oxford
local.contributor.affiliationvon Caemmerer, Susanne, College of Science, ANU
local.contributor.affiliationFurbank, Robert, College of Science, ANU
dc.relationhttp://purl.org/au-research/grants/arc/CE140100015
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage1135
local.bibliographicCitation.lastpage1145
local.identifier.doi10.1093/jxb/erx456
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2019-03-31T07:24:00Z
local.identifier.scopusID2-s2.0-85042590889
dcterms.accessRightsOpen Access
dc.provenanceThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.licenseCreative Commons Attribution License
CollectionsANU Research Publications

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