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De Novo Transcriptome Study Identifies Candidate Genes Involved in Resistance to Austropuccinia psidii (Myrtle Rust) in Syzygium luehmannii (Riberry)

Tobias, P.A.; Guest, David I.; Kulheim, Carsten; Park, Robert

Description

Austropuccinia psidii, causal agent of myrtle rust, was discovered in Australia in 2010 and has since become established on a wide range of species within the family Myrtaceae. Syzygium luehmannii, endemic to Australia, is an increasingly valuable berry crop. Plants were screened for responses to A. psidii inoculation, and specific resistance, in the form of localized necrosis, was determined in 29% of individuals. To understand the molecular basis underlying this response, mRNA was sequenced...[Show more]

dc.contributor.authorTobias, P.A.
dc.contributor.authorGuest, David I.
dc.contributor.authorKulheim, Carsten
dc.contributor.authorPark, Robert
dc.date.accessioned2019-07-29T06:05:26Z
dc.identifier.issn0031-949X
dc.identifier.urihttp://hdl.handle.net/1885/164772
dc.description.abstractAustropuccinia psidii, causal agent of myrtle rust, was discovered in Australia in 2010 and has since become established on a wide range of species within the family Myrtaceae. Syzygium luehmannii, endemic to Australia, is an increasingly valuable berry crop. Plants were screened for responses to A. psidii inoculation, and specific resistance, in the form of localized necrosis, was determined in 29% of individuals. To understand the molecular basis underlying this response, mRNA was sequenced from leaf samples taken preinoculation, and at 24 and 48 h postinoculation, from four resistant and four susceptible plants. Analyses, based on de novo transcriptome assemblies for all plants, identified significant expression changes in resistant plants (438 transcripts) 48 h after pathogen exposure compared with susceptible plants (three transcripts). Most significantly up-regulated in resistant plants were gene homologs for transcription factors, receptor-like kinases, and enzymes involved in secondary metabolite pathways. A putative G-type lectin receptor-like kinase was exclusively expressed in resistant individuals and two transcripts incorporating toll/interleukin-1, nucleotide binding site, and leucine-rich repeat domains were up-regulated in resistant plants. The results of this study provide the first early gene expression profiles for a plant of the family Myrtaceae in response to the myrtle rust pathogen.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherAmerican Phytopathological Society
dc.rights© 2018 The American Phytopathological Society
dc.sourcePhytopathology
dc.titleDe Novo Transcriptome Study Identifies Candidate Genes Involved in Resistance to Austropuccinia psidii (Myrtle Rust) in Syzygium luehmannii (Riberry)
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume108
dc.date.issued2018
local.identifier.absfor060704 - Plant Pathology
local.identifier.ariespublicationu4485658xPUB2063
local.publisher.urlhttps://www.apsnet.org/Pages/default.aspx
local.type.statusPublished Version
local.contributor.affiliationTobias, P.A., University of Sydney
local.contributor.affiliationGuest, David I., University of Sydney
local.contributor.affiliationKulheim, Carsten, College of Science, ANU
local.contributor.affiliationPark, Robert, University of Sydney
local.description.embargo2037-12-31
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage627
local.bibliographicCitation.lastpage640
local.identifier.doi10.1094/PHYTO-09-17-0298-R
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2019-03-31T07:23:02Z
local.identifier.scopusID2-s2.0-85046027791
local.identifier.thomsonID000430497700009
CollectionsANU Research Publications

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