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Biochemical characterization and chemical inhibition o PfATP4-associated Na+-ATPase activity in Plasmodium falciparum membranes

Rosling, James; Ridgway, Melanie; Summers, Robert; Kirk, Kiaran; Lehane, Adele

Description

The antimalarial activity of chemically diverse compounds, including the clinical candidate cipargamin, has been linked to the ATPase PfATP4 in the malaria-causing parasite Plasmodium falciparum. The characterization of PfATP4 has been hampered by the inability thus far to achieve its functional expression in a heterologous system. Here, we optimized a membrane ATPase assay to probe the function of PfATP4 and its chemical sensitivity. We found that cipargamin inhibited the Na+-dependent ATPase...[Show more]

CollectionsANU Research Publications
Date published: 2018-07-09
Type: Journal article
URI: http://hdl.handle.net/1885/164737
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.RA118.003640
Access Rights: Open Access

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