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Diverse peptide hormones affecting root growth identified in the Medicago truncatula secreted peptidome

Patel, Neha; Mohd Radzman, Nadiatul; Corcilius, Leo; Crossett, Ben; Connolly, Angela; Cordwell, Stuart; Ivanovici, Ariel; Taylor, Katia; Willams, James; Binos, Steve; Mariani, Michael; Payne, Richard J; Djordjevic, Michael

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Multigene families encoding diverse secreted peptide hormones play important roles in plant development. A need exists to efficiently elucidate the structures and post-translational-modifications of these difficult-to-isolate peptide hormones in planta so that their biological functions can be determined. A mass spectrometry and bioinformatics approach was developed to comprehensively analyze the secreted peptidome of Medicago hairy root cultures and xylem sap. We identified 759 spectra...[Show more]

dc.contributor.authorPatel, Neha
dc.contributor.authorMohd Radzman, Nadiatul
dc.contributor.authorCorcilius, Leo
dc.contributor.authorCrossett, Ben
dc.contributor.authorConnolly, Angela
dc.contributor.authorCordwell, Stuart
dc.contributor.authorIvanovici, Ariel
dc.contributor.authorTaylor, Katia
dc.contributor.authorWillams, James
dc.contributor.authorBinos, Steve
dc.contributor.authorMariani, Michael
dc.contributor.authorPayne, Richard J
dc.contributor.authorDjordjevic, Michael
dc.date.accessioned2019-07-10T01:33:05Z
dc.date.available2019-07-10T01:33:05Z
dc.identifier.issn1535-9476
dc.identifier.urihttp://hdl.handle.net/1885/164520
dc.description.abstractMultigene families encoding diverse secreted peptide hormones play important roles in plant development. A need exists to efficiently elucidate the structures and post-translational-modifications of these difficult-to-isolate peptide hormones in planta so that their biological functions can be determined. A mass spectrometry and bioinformatics approach was developed to comprehensively analyze the secreted peptidome of Medicago hairy root cultures and xylem sap. We identified 759 spectra corresponding to the secreted products of twelve peptide hormones including four CEP (C-TERMINALLY ENCODED PEPTIDE), two CLE (CLV3/ENDOSPERM SURROUNDING REGION RELATED) and six XAP (XYLEM SAP ASSOCIATED PEPTIDE) peptides. The MtCEP1, MtCEP2, MtCEP5 and MtCEP8 peptides identified differed in post-translational-modifications. Most were hydroxylated at conserved proline residues but some MtCEP1 derivatives were tri-arabinosylated. In addition, many CEP peptides possessed unexpected N- and C-terminal extensions. The pattern of these extensions suggested roles for endo- and exoproteases in CEP peptide maturation. Longer than expected, hydroxylated and homogeneously modified mono- and tri-arabinosylated CEP peptides corresponding to their in vivo structures were chemically synthesized to probe the effect of these post-translational-modifications on function. The ability of CEP peptides to elevate root nodule number was increased by hydroxylation at key positions. MtCEP1 peptides with N-terminal extensions or with tri-arabinosylation modification, however, were unable to impart increased nodulation. The MtCLE5 and MtCLE17 peptides identified were of precise size, and inhibited main root growth and increased lateral root number. Six XAP peptides, each beginning with a conserved DY sulfation motif, were identified including MtXAP1a, MtXAP1b, MtXAP1c, MtXAP3, MtXAP5 and MtXAP7. MtXAP1a and MtXAP5 inhibited lateral root emergence. Transcriptional analyses demonstrated peptide hormone gene expression in the root vasculature and tip. Since hairy roots can be induced on many plants, their corresponding root cultures may represent ideal source materials to efficiently identify diverse peptide hormones in vivo in a broad range of species.
dc.description.sponsorshipThis work was supported by ARC grants to MAD: DP150104050 and LP150100826. NP was partly supported by an Endeavor Fellowship. NAMR was supported by an ANU Ph.D. scholarship supported by DP120101893. AI was supported by an Australian Post-graduate Award and an AW Howard Memorial Award. LC was supported by the Bruce-Veness Chandler and the John A. Lamberton research scholarships
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.rights© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.sourceMolecular and Cellular Proteomics
dc.titleDiverse peptide hormones affecting root growth identified in the Medicago truncatula secreted peptidome
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume17
dc.date.issued2018-01-01
local.identifier.absfor060702 - Plant Cell and Molecular Biology
local.identifier.absfor060705 - Plant Physiology
local.identifier.absfor030406 - Proteins and Peptides
local.identifier.ariespublicationa383154xPUB9259
local.publisher.urlhttp://www.asbmb.org/
local.type.statusPublished Version
local.contributor.affiliationPatel, Neha, College of Science, ANU
local.contributor.affiliationMohd Radzman, Nadiatul, College of Science, ANU
local.contributor.affiliationCorcilius, Leo, The University of Sydney
local.contributor.affiliationCrossett, Ben, The University of Sydney,
local.contributor.affiliationConnolly, Angela, University of Sydney
local.contributor.affiliationCordwell, Stuart, University of Sydney
local.contributor.affiliationIvanovici, Ariel, College of Science, ANU
local.contributor.affiliationTaylor, Katia, College of Science, ANU
local.contributor.affiliationWillams, James, The University of Sydney,
local.contributor.affiliationBinos, Steve, Thermo Fisher Scientific Pty. Ltd
local.contributor.affiliationMariani, Michael, Thermo Fisher Scientific Pty. Ltd
local.contributor.affiliationPayne, Richard J, University of Sydney
local.contributor.affiliationDjordjevic, Michael, College of Science, ANU
dc.relationhttp://purl.org/au-research/grants/arc/DP150104050
dc.relationhttp://purl.org/au-research/grants/arc/LP150100826
dc.relationhttp://purl.org/au-research/grants/arc/DP120101893
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage160
local.bibliographicCitation.lastpage174
local.identifier.doi10.1074/mcp.RA117.000168
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.absseo970107 - Expanding Knowledge in the Agricultural and Veterinary Sciences
dc.date.updated2019-03-31T07:18:24Z
local.identifier.scopusID2-s2.0-85040242809
dcterms.accessRightsOpen Access
dc.provenancehttp://sherpa.ac.uk/romeo/issn/1535-9476/..."Publisher's version/PDF may be used after a 12 months embargo period" from SHERPA/RoMEO site (as at 10/07/19).
CollectionsANU Research Publications

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