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Alternate steroid sulfation pathways targeted by LC-MS/MS analysis of disulfates: Application to prenatal diagnosis of steroid synthesis disorders

Pozo, Oscar J; Marcos, Josep; Khymenets, Olha; Pranata, Andy; Fitzgerald, Christopher; McLeod, Malcolm; Shackleton, Cedric


The steroid disulfates (aka bis-sulfates) are a significant but minor fraction of the urinary steroid metabolome that have not been widely studied because major components are not hydrolyzed by the commercial sulfatases commonly used in steroid metabolomics. In early studies, conjugate fractionation followed by hydrolysis using acidified solvent (solvolysis) was used for the indirect detection of this fraction by GC–MS. This paper describes the application of a specific LC–MS/MS method for the...[Show more]

CollectionsANU Research Publications
Date published: 2018
Type: Journal article
Source: Journal of Molecular Endocrinology
DOI: 10.1530/JME-17-0286


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