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An improved measurement of the Ca²⁺-binding affinity of fluorescent Ca²⁺ indicators

Tran, Le-Thuy-Van; Park, Chang Ha Michael; Stricker, Christian

Description

Fluorescent Ca2+ indicators are widely used to measure the intracellular Ca2+ concentration ([Ca2+]i) in living cells, including neurons. By calibrating an indicator in solutions that mimic the main ionic constituents of the actual cytoplasm, [Ca2+]i can be determined from the measured fluorescence intensity. However, different studies have reported considerably different Ca2+-binding affinities (Kd) for the same indicator, even though they used calibrating solutions with similar compositions....[Show more]

CollectionsANU Research Publications
Date published: 2018
Type: Journal article
URI: http://hdl.handle.net/1885/163979
Source: Cell Calcium
DOI: 10.1016/j.ceca.2018.01.001

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