Orton, Henry; Otting, Gottfried
Measurements of paramagnetic relaxation enhancements (PREs) in 1H NMR spectra are an important tool to obtain long-range distance information in proteins, but quantitative interpretation is easily compromised by nonspecific intermolecular PREs. Here we show that PREs generated by lanthanides with anisotropic magnetic susceptibilities offer a route to accurate calibration-free distance measurements. As these lanthanides change 1H chemical shifts due to pseudocontact shifts, the relaxation rates...[Show more]
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.