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Modeling the fitness consequences of a cyanophage-encoded photosynthesis gene

Bragg, Jason G.; Chisholm, Sallie W.

Description

BACKGROUND Phages infecting marine picocyanobacteria often carry a psbA gene, which encodes a homolog to the photosynthetic reaction center protein, D1. Host encoded D1 decays during phage infection in the light. Phage encoded D1 may help to maintain photosynthesis during the lytic cycle, which in turn could bolster the production of deoxynucleoside triphosphates (dNTPs) for phage genome replication. METHODOLOGY/PRINCIPAL FINDINGS To explore the consequences to a phage of encoding and...[Show more]

dc.contributor.authorBragg, Jason G.
dc.contributor.authorChisholm, Sallie W.
dc.date.accessioned2015-10-28T00:53:36Z
dc.date.available2015-10-28T00:53:36Z
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1885/16141
dc.description.abstractBACKGROUND Phages infecting marine picocyanobacteria often carry a psbA gene, which encodes a homolog to the photosynthetic reaction center protein, D1. Host encoded D1 decays during phage infection in the light. Phage encoded D1 may help to maintain photosynthesis during the lytic cycle, which in turn could bolster the production of deoxynucleoside triphosphates (dNTPs) for phage genome replication. METHODOLOGY/PRINCIPAL FINDINGS To explore the consequences to a phage of encoding and expressing psbA, we derive a simple model of infection for a cyanophage/host pair--cyanophage P-SSP7 and Prochlorococcus MED4--for which pertinent laboratory data are available. We first use the model to describe phage genome replication and the kinetics of psbA expression by host and phage. We then examine the contribution of phage psbA expression to phage genome replication under constant low irradiance (25 microE m(-2) s(-1)). We predict that while phage psbA expression could lead to an increase in the number of phage genomes produced during a lytic cycle of between 2.5 and 4.5% (depending on parameter values), this advantage can be nearly negated by the cost of psbA in elongating the phage genome. Under higher irradiance conditions that promote D1 degradation, however, phage psbA confers a greater advantage to phage genome replication. CONCLUSIONS/SIGNIFICANCE These analyses illustrate how psbA may benefit phage in the dynamic ocean surface mixed layer.
dc.description.sponsorshipThis work was supported in part by funds from the US National Science Foundation, the Gordon and Betty Moore Foundation Marine Microbiology Initiative, and the US Department of Energy GTL Program. It is an NSF C-MORE contribution.
dc.format9 pages
dc.publisherPublic Library of Science
dc.rights© 2008 Bragg et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.sourcePLoS ONE
dc.subjectadaptation, biological
dc.subjectbacteriophages
dc.subjectcomputational biology
dc.subjectcyanobacteria
dc.subjectdna replication
dc.subjectgene expression regulation, viral
dc.subjectgene knockout techniques
dc.subjectgenome, viral
dc.subjectphotosynthesis
dc.subjectseawater
dc.subjectmodels, theoretical
dc.titleModeling the fitness consequences of a cyanophage-encoded photosynthesis gene
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume3
dcterms.dateAccepted2008-10-03
dc.date.issued2008-10-29
local.identifier.absfor060303
local.identifier.ariespublicationU3488905xPUB992
local.publisher.urlhttps://www.plos.org/
local.type.statusPublished Version
local.contributor.affiliationBragg, Jason, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Evolution, Ecology & Genetics, The Australian National University
local.contributor.affiliationChisholm, Sallie W., Massachusetts Institute of Technology, United States of America
local.identifier.essn1932-6203
local.bibliographicCitation.issue10
local.bibliographicCitation.startpagee3550
local.identifier.doi10.1371/journal.pone.0003550
local.identifier.absseo960805
dc.date.updated2015-12-10T09:46:57Z
local.identifier.scopusID2-s2.0-56149118576
local.identifier.thomsonID000265131100009
CollectionsANU Research Publications

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