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Intramolecular epistasis and the evolution of a new enzymatic function

Noor, Sajid; Taylor, Matthew C; Russell, Robyn J; Jermiin, Lars S; Jackson, Colin J; Oakeshott, John Graham; Scott, Colin


Atrazine chlorohydrolase (AtzA) and its close relative melamine deaminase (TriA) differ by just nine amino acid substitutions but have distinct catalytic activities. Together, they offer an informative model system to study the molecular processes that underpin the emergence of new enzymatic function. Here we have constructed the potential evolutionary trajectories between AtzA and TriA, and characterized the catalytic activities and biophysical properties of the intermediates along those...[Show more]

CollectionsANU Research Publications
Date published: 2012-06-29
Type: Journal article
Source: PLoS ONE
DOI: 10.1371/journal.pone.0039822


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