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A 5000-fold increase in the specificity of a bacterial Phosphotriesterase for malathion through combinatorial active site mutagenesis

Naqvi, Tatheer; Warden, Andrew C; French, Nigel; Sugrue, Elena; Carr, Paul D; Jackson, Colin; Scott, Colin


Phosphotriesterases (PTEs) have been isolated from a range of bacterial species, including Agrobcaterium radiobacter (PTEAr), and are efficient enzymes with broad substrate ranges. The turnover rate of PTEAr for the common organophosphorous insecticide malathion is lower than expected based on its physical properties; principally the pka of its leaving group. In this study, we rationalise the turnover rate of PTEAr for malathion using computational docking of the substrate into a high...[Show more]

CollectionsANU Research Publications
Date published: 2014-04-10
Type: Journal article
Source: PLoS ONE
DOI: 10.1371/journal.pone.0094177
Access Rights: Open Access


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