Field-caught permethrin-resistant anopheles gambiae overexpress CYP6P3, a P450 that metabolises pyrethroids
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Müller, Pie; Warr, Emma; Stevenson, Bradley J.; Pignatelli, Patricia M.; Morgan, John C.; Steven, Andrew; Yawson, Alexander E.; Mitchell, Sara N.; Ranson, Hilary; Hemingway, Janet; Paine, Mark J. I.; Donnelly, Martin J.
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Insects exposed to pesticides undergo strong natural selection and have developed various adaptive mechanisms to survive. Resistance to pyrethroid insecticides in the malaria vector Anopheles gambiae is receiving increasing attention because it threatens the sustainability of malaria vector control programs in sub-Saharan Africa. An understanding of the molecular mechanisms conferring pyrethroid resistance gives insight into the processes of evolution of adaptive traits and facilitates the...[Show more]
dc.contributor.author | Müller, Pie | |
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dc.contributor.author | Warr, Emma | |
dc.contributor.author | Stevenson, Bradley J. | |
dc.contributor.author | Pignatelli, Patricia M. | |
dc.contributor.author | Morgan, John C. | |
dc.contributor.author | Steven, Andrew | |
dc.contributor.author | Yawson, Alexander E. | |
dc.contributor.author | Mitchell, Sara N. | |
dc.contributor.author | Ranson, Hilary | |
dc.contributor.author | Hemingway, Janet | |
dc.contributor.author | Paine, Mark J. I. | |
dc.contributor.author | Donnelly, Martin J. | |
dc.date.accessioned | 2015-10-27T00:49:59Z | |
dc.date.available | 2015-10-27T00:49:59Z | |
dc.identifier.issn | 1553-7404 | |
dc.identifier.uri | http://hdl.handle.net/1885/16111 | |
dc.description.abstract | Insects exposed to pesticides undergo strong natural selection and have developed various adaptive mechanisms to survive. Resistance to pyrethroid insecticides in the malaria vector Anopheles gambiae is receiving increasing attention because it threatens the sustainability of malaria vector control programs in sub-Saharan Africa. An understanding of the molecular mechanisms conferring pyrethroid resistance gives insight into the processes of evolution of adaptive traits and facilitates the development of simple monitoring tools and novel strategies to restore the efficacy of insecticides. For this purpose, it is essential to understand which mechanisms are important in wild mosquitoes. Here, our aim was to identify enzymes that may be important in metabolic resistance to pyrethroids by measuring gene expression for over 250 genes potentially involved in metabolic resistance in phenotyped individuals from a highly resistant, wild A. gambiae population from Ghana. A cytochrome P450, CYP6P3, was significantly overexpressed in the survivors, and we show that the translated enzyme metabolises both alpha-cyano and non-alpha-cyano pyrethroids. This is the first study to demonstrate the capacity of a P450 identified in wild A. gambiae to metabolise insecticides. The findings add to the understanding of the genetic basis of insecticide resistance in wild mosquito populations. | |
dc.description.sponsorship | The study was funded by the Innovative Vector Control Consortium. | |
dc.format | 10 pages | |
dc.publisher | Public Library of Science | |
dc.rights | © 2008 Muller et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.source | PLoS Genetics | |
dc.subject | animals | |
dc.subject | anopheles gambiae | |
dc.subject | cloning, molecular | |
dc.subject | cytochrome p-450 enzyme system | |
dc.subject | escherichia coli | |
dc.subject | genotype | |
dc.subject | insect proteins | |
dc.subject | insecticide resistance | |
dc.subject | insecticides | |
dc.subject | permethrin | |
dc.title | Field-caught permethrin-resistant anopheles gambiae overexpress CYP6P3, a P450 that metabolises pyrethroids | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 4 | |
dcterms.dateAccepted | 2008-10-29 | |
dc.date.issued | 2008-11-28 | |
local.identifier.absfor | 030499 | |
local.identifier.ariespublication | u4005981xPUB786 | |
local.publisher.url | https://www.plos.org/ | |
local.type.status | Published Version | |
local.contributor.affiliation | Muller, Pie, Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Warr, Emma, Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Stevenson, Bradley, College of Physical and Mathematical Sciences, CPMS Research School of Chemistry, RSC General, The Australian National University | |
local.contributor.affiliation | Pignatelli, Patricia, Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Morgan, John C., Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Steven, Andrew, Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Egyir-Yawson, Alexander, Ghana Atomic Energy Commission, Ghana | |
local.contributor.affiliation | Mitchell, Sarah N., Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Ranson, Hilary, Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Hemingway, Janet, Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Paine, Mark J. I., Liverpool School of Tropical Medicine, United Kingdom | |
local.contributor.affiliation | Donnelly, Martin James, Liverpool School of Tropical Medicine, United Kingdom | |
local.identifier.essn | 1553-7404 | |
local.bibliographicCitation.issue | 11 | |
local.bibliographicCitation.startpage | e1000286 | |
local.bibliographicCitation.lastpage | 10 | |
local.identifier.doi | 10.1371/journal.pgen.1000286 | |
local.identifier.absseo | 970106 | |
dc.date.updated | 2015-12-10T09:08:42Z | |
local.identifier.scopusID | 2-s2.0-57149115769 | |
Collections | ANU Research Publications |
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